GLUCOSE SYNTHESIS IN TRITIATED-WATER
GLUCOSE SYNTHESIS IN TRITIATED-WATER
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DOI：
10.1111/j.1432-1033.1974.tb03703.x
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发表时间：
1974-01-01
期刊：
影响因子：
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通讯作者：
KATZ, J
中科院分区：
文献类型：
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作者：
ROGNSTAD, R;CLARK, DG;KATZ, J
分离的大鼠或仓鼠肝实质细胞或肾皮质节段在含3 HHO的培养基中孵育，并测定氚在所形成的葡萄糖中的掺入和部分分布。来自乳酸或丙酮酸的葡萄糖异生作用导致约80%至90%的可能量（每个葡萄糖分子7 μ原子）的氚从3 HHO固定在葡萄糖中，其中C-6标记的百分比很高。在厌氧条件下，糖原分解形成的葡萄糖导致了不到20%的氚固定，大部分标记在C-2上。这种技术可能最终用于确定葡萄糖形成的速率和来源。
Isolated rat or hamster liver parenchymal cells or kidney cortex segments were incubated in a medium containing3HHO and the incorporation and partial distribution of tritium in the glucose formed was determined. Gluconeogenesis from lactate or pyruvate caused about 80 to 90% of the possible amount (7 μatoms per glucose molecule) of fixation of tritium from3HHO in glucose, with a high percentage labelling on C‐6. Glucose formation from glycogen breakdown under anaerobiosis caused less than 20% of the possible fixation of tritium, with most of the labelling on C‐2. This technique may be of eventual use in determining rates and sources of glucose formation.