Analysis on the complete genome of a novel caprine parainfluenza virus 3.
Analysis on the complete genome of a novel caprine parainfluenza virus 3.
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DOI：
10.1016/j.meegid.2015.11.027
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发表时间：
2016-03
期刊：
影响因子：
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通讯作者：
Leilei Yang;Wenliang Li;Li-Guo Mao;F. Hao;Zhongyu Wang;Wenwen Zhang;Jiawu Deng;Jieyuan Jiang-Jieyuan-Jia
中科院分区：
文献类型：
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作者：
Leilei Yang;Wenliang Li;Li-Guo Mao;F. Hao;Zhongyu Wang;Wenwen Zhang;Jiawu Deng;Jieyuan Jiang-Jieyuan-Jia
3型副流感病毒（PIV3）是人类和许多动物最重要的病毒性呼吸道病原体之一。2013年在中国山羊呼吸道疾病群中分离到一株独特的CPIV3 （CPIV3）菌株JS2013。至此，菌株JS2013的全基因组序列已经确定。引物步行RT-PCR共获得15个重叠的DNA克隆，覆盖了病毒的整个基因组。用3′和5′RACE扩增病毒基因组3′和5′端序列。病毒基因组全长15618个核苷酸（nt），由6个基因组成，顺序为5′-leader-N-P/C/ v - m - f - hn -l -tail -3′。两个基因之间的连接序列是高度保守的基因启动和停止信号序列，以及与其他报道的PIV3株相似的三核苷酸基因间区（IGR）。基于JS2013与其他呼吸道病毒属病毒株全基因组的系统发育分析表明，JS2013与HPIV1、仙台病毒、HPIV3和其他已报道的BPIV3基因型存在明显差异。进一步分析JS2013的HN基因以及随后分离的另外两株CPIV3菌株表明，CPIV3菌株形成了一个独立的聚类。本研究结果表明，CPIV3是呼吸道病毒属的新成员。
Parainfluenza virus type 3 (PIV3) is one of the most important viral respiratory pathogens for humans and for many animals. One unique caprine PIV3 (CPIV3) strain named JS2013 was isolated in Chinese goat flocks with respiratory diseases in 2013. Now, the complete genome sequence of the strain JS2013 had been determined. A total of 15 overlapping DNA clones, covering the entire genome of the virus, were obtained by primer walking RT-PCR. The sequences of the 3′ and 5′ termini of the viral genome were amplified by 3′ and 5′ RACE. The viral genome was 15,618 nucleotides (nt) in length, which was consisted of six genes in the order 5′-leader-N-P/C/V-M-F-HN-L-tailer-3′. The junction sequences between two genes were highly conserved gene start and stop signal sequences, and trinucleotide intergenic regions (IGR) similar to those of other reported PIV3 strains. Phylogenetic analysis based on the complete genomes of JS2013 with other strains of genus Respirovirus demonstrated that the JS2013 obviously differed from HPIV1, Sendai virus, HPIV3 and other reported BPIV3 genotypes. Further analysis of HN genes of JS2013 along with two more CPIV3 strains isolated later indicated that CPIV3 strains formed a separate cluster. The results presented here suggested that CPIV3 is a new member of the genusRespirovirus.