Recombinant Lactococcus lactis co-expressing dendritic cell target peptide and E. tenella 3-1E protein: immune response and efficacy against homologous challenge
Recombinant Lactococcus lactis co-expressing dendritic cell target peptide and E. tenella 3-1E protein: immune response and efficacy against homologous challenge
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DOI：
10.1080/09540105.2020.1733495
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发表时间：
2020-01
影响因子：
3
通讯作者：
Guanghao Li;Chunli Ma;Dian Wang;Wenjing Chen;D. Ma
中科院分区：
文献类型：
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作者：
Guanghao Li;Chunli Ma;Dian Wang;Wenjing Chen;D. Ma
摘要本研究构建了携带锚定树突状细胞靶向多肽(DCpep)和柔嫩艾美耳球虫3-1E蛋白的重组乳酸乳杆菌NZ9000。用重组乳球菌口服免疫雏鸡，每隔2周免疫3次。三次免疫后，用柔嫩艾美耳球虫孢子化卵囊攻击鸡，评价免疫应答和保护效果。动物实验发现，与表达3-1E蛋白的重组乳球菌相比，表达锚定DCpep-3-1E蛋白的重组乳球菌能诱导更高水平的3-1E特异性血清免疫球蛋白和盲肠灌洗液中分泌型免疫球蛋白A(SIgA)，外周血中CD_4~+和CD_8α~+细胞比例以及脾中IL-2和干扰素-γ的m RNA表达水平均高于表达3-1E蛋白的乳球菌，从而对同源攻击具有明显的保护作用。本工作表明，乳球菌细胞表面展示的靶蛋白和免疫增强剂可能是一种有前景的增强免疫和对抗病原体感染的免疫效果的方法。
ABSTRACT In the present study, recombinant L. lactis NZ9000 displaying anchored dendritic cell-targeting peptide (DCpep) and E. tenella 3-1E protein was constructed. Chickens were orally immunized with the recombinant lactococci for three times at 2-week intervals. After three immunizations, chickens were challenged with E. tenella sporulated oocyst, and the immune response and protective efficacy were evaluated. The finding from animal experiments showed recombinant lactococci expressing anchored DCpep-3-1E protein elicited higher levels of 3-1E specific serum IgG and secretory IgA (sIgA) in caecal lavage, higher proportions of CD4+ and CD8α+ cells in peripheral blood, and higher mRNA expression levels of IL-2 and IFN-γ in the spleen compared to 3-1E-expressing lactococci, and hence offered obvious protective efficacy against homologous challenge. The present work demonstrated that cell surface-displayed target protein and immune enhancers in lactococci may be a promising approach to enhance immunity and immune efficacy against pathogen infection.