iTRAQ-based proteomic analysis on the mitochondrial responses in gill tissues of juvenile olive flounder Paralichthys olivaceus exposed to cadmium
iTRAQ-based proteomic analysis on the mitochondrial responses in gill tissues of juvenile olive flounder Paralichthys olivaceus exposed to cadmium
复制标题
基于 iTRAQ 的蛋白质组学分析对镉暴露的牙鲆幼鱼鳃组织线粒体反应的影响
DOI：
10.1016/j.envpol.2019.113591
复制
发表时间：
2020-02-01
影响因子：
8.9
通讯作者：
Wu, Huifeng
中科院分区：
文献类型：
--
作者：
Lu, Zhen;Wang, Shuang;Wu, Huifeng
镉（Cd）是渤海海域重要的重金属污染物。线粒体被认为是镉毒性的关键靶点。然而,线粒体!海洋鱼类对镉的反应尚未得到充分调查。本研究通过测定线粒体膜电位（MMP）、线粒体形态观察和定量蛋白质组学分析，研究了两种环境相关浓度（5和50 μ g/L） Cd处理14 d后，橄榄鲆幼鱼（Paralichthys olivaceus）鳃线粒体反应。两种Cd处理均显著降低了比目鱼鳃线粒体的MMPs。cd处理后的比目鱼线粒体形态发生改变，线粒体增多，线粒体变小。基于itraq的蛋白质组学分析表明，在两种Cd处理中，共有128个蛋白存在差异表达。这些蛋白基本参与鳃线粒体的多种生物过程，包括线粒体形态和输入、三羧酸（TCA）循环、氧化磷酸化（OXPHOS）、初级胆汁酸生物合成、抗逆性和凋亡。这些结果表明，鳃线粒体的能量稳态、胆固醇代谢、抗逆性、细胞凋亡和线粒体形态的动态调节可能在Cd毒性反应中起重要作用。总的来说，本研究利用itraq为基础的蛋白质组学研究提供了镉在比目鱼鳃线粒体毒性的全局视图。(C) 2019 Elsevier Ltd.版权所有。
Cadmium (Cd) is an important heavy metal pollutant in the Bohai Sea. Mitochondria are recognized as the key target for Cd toxicity. However, mitochondria! responses to Cd have not been fully investigated in marine fishes. In this study, the mitochondrial responses were characterized in gills of juvenile flounder Paralichthys olivaceus treated with two environmentally relevant concentrations (5 and 50 mu g/L) of Cd for 14 days by determination of mitochondrial membrane potential (MMP), observation of mitochondrial morphology and quantitative proteomic analysis. Both Cd treatments significantly decreased MMPs of mitochondria from flounder gills. Mitochondrial morphologies were altered in Cd-treated flounder samples, indicated by more and smaller mitochondria. iTRAQ-based proteomic analysis indicated that a total of 128 proteins were differentially expressed in both Cd treatments. These proteins were basically involved in various biological processes in gill mitochondria, including mitochondrial morphology and import, tricarboxylic acid (TCA) cycle, oxidative phosphorylation (OXPHOS), primary bile acid biosynthesis, stress resistance and apoptosis. These results indicated that dynamic regulations of energy homeostasis, cholesterol metabolism, stress resistance, apoptosis, and mitochondrial morphology in gill mitochondria might play significant roles in response to Cd toxicity. Overall, this study provided a global view on mitochondrial toxicity of Cd in flounder gills using iTRAQ-based proteomics. (C) 2019 Elsevier Ltd. All rights reserved.