ANATOMY OF HERPES-SIMPLEX VIRUS DNA - EVIDENCE FOR 4 POPULATIONS OF MOLECULES THAT DIFFER IN RELATIVE ORIENTATIONS OF THEIR LONG AND SHORT COMPONENTS .4.
ANATOMY OF HERPES-SIMPLEX VIRUS DNA - EVIDENCE FOR 4 POPULATIONS OF MOLECULES THAT DIFFER IN RELATIVE ORIENTATIONS OF THEIR LONG AND SHORT COMPONENTS .4.
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DOI：
10.1073/pnas.72.11.4243
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发表时间：
1975-01-01
影响因子：
11.1
通讯作者：
ROIZMAN, B
中科院分区：
文献类型：
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作者：
HAYWARD, GS;JACOB, RJ;ROIZMAN, B
从 HSV-1（单纯疱疹病毒 1、人类疱疹病毒 1）MP 病毒株中提取的完整 DNA 分子的分子量约为 97 X 10(6)，但用 HinIII 限制性酶切割会产生 14 个片段，总分子量为 160 X 10(6)。六个“主要”片段在群体中的每个分子中出现一次，占遗传信息的 60%。四个“次要”片段的存在量相当于每两个基因组一个拷贝（0.5 摩尔比），而其他四个片段仅每四个分子出现一次（0.25 摩尔比）。小片段可以排列成四个等摩尔组，每个组的总分子量占基因组剩余 40%。用 lambda 5' 核酸外切酶处理表明所有分子在两个末端都含有 0.5 摩尔比的片段。这些观察结果以及对 EcoRI 和双 HinIII/EcoRI 消化物的类似分析结果表明，HSV DNA 有四种不同的结构形式，它们仅在两个亚区域（称为 L 和 S）的相对方向上有所不同。L 和 S 片段分别由 82% 和 18% 的序列组成，并且每个片段都具有与电子显微镜观察到的内部重复相对应的反向末端冗余区域。来自其他 HSV-1 和 2 毒株的 DNA 也由相同比例的 L 和 S 片段的所有四种可能排列组成。 HSV DNA 分子的这些不寻常特征对于单纯疱疹病毒的遗传图谱以及复制和进化模式具有新的意义。
Intact DNA molecules extracted from HSV-1 (herpes simplex virus 1, human herpes virus 1) strain MP virions have a molecular weight of approximately 97 X 10(6), but cleavage with the HinIII restriction enzyme yields fourteen fragments with summed molecular weights of 160 X 10(6). Six "major" fragments occur once in every molecule in the population and account for 60% of the genetic information. Four "minor" fragments are present in amounts equivalent to one copy for every two genomes (0.5 molar ratio) and the other four occur only once in every four molecules (0.25 molar ratio). The minor fragments can be arranged into four equimolar sets, each with summed molecular weights that account for the remaining 40% of the genome. Treatment with lambda 5' exonuclease revealed that all molecules contain 0.5 molar ratio fragments at both termini. These observations and the results of similar analyses of the EcoRI and double HinIII/EcoRI digests indicate that there are four distinct structural forms of HSV DNA which differ only in the relative orientations of two subregions, designated L and S. The L and S segments consist of 82 and 18% of the sequences, respectively, and each has inverted terminally redundant regions that correspond to the internal duplications observed by electron microscopy. The DNA from other strains of HSV-1 and 2 also consists of equal proportions of all four possible permutations of the L and S segments. These unusual features of HSV DNA molecules have novel implications with regard to the genetic map and the mode of replication and evolution of herpes simples viruses.