Direct Adventitious Shoot Formation from Apical Shoot Explants of Euphorbia tirucalli
Direct Adventitious Shoot Formation from Apical Shoot Explants of Euphorbia tirucalli
复制标题
DOI：
10.1007/s00344-010-9170-z
复制
发表时间：
2011-03
影响因子：
4.8
通讯作者：
G. Ma;J. A. Teixeira da Silva;Guojiang Wu
中科院分区：
文献类型：
--
作者：
G. Ma;J. A. Teixeira da Silva;Guojiang Wu
以绿大戟茎尖为外植体，研究了不定芽的诱导。在MS（Murashige和Skoog）培养基中，添加0.5 mg l− 1噻苯隆和0.5 mg l − 1苄氨基嘌呤，平均可在一个顶端外植体的节段上有效诱导10.5个不定芽。不定芽在无菌MS培养基上继续培养可分化为不定芽。为了生根，将芽丛培养在含有0.2 mg l−1α-萘乙酸或吲哚-3-丁酸的半强度MS培养基上。所有生根植株在2个月内都能成活。
The induction of adventitious buds from apical shoot explants ofEuphorbia tirucalliwas studied. On average, 10.5 adventitious buds were efficiently induced in a ring on the segment from one apical explant on MS (Murashige and Skoog) medium supplemented with 0.5 mg l−1thidiazuron and 0.5 mg l−1benzylaminopurine. The adventitious buds could develop into adventitious shoots during subsequent cultures on hormone-free MS medium. For rooting, shoot clumps were cultured on half-strength MS medium containing 0.2 mg l−1α-naphthaleneacetic acid or indole-3-butyric acid. All the rooted plants survived establishment in soil within 2 months.