Transmembrane Proteins UNC-40/DCC, PTP-3/LAR, and MIG-21 Control Anterior–Posterior Neuroblast Migration with Left–Right Functional Asymmetry in Caenorhabditis elegans
Transmembrane Proteins UNC-40/DCC, PTP-3/LAR, and MIG-21 Control Anterior–Posterior Neuroblast Migration with Left–Right Functional Asymmetry in Caenorhabditis elegans
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DOI：
10.1534/genetics.112.145706
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发表时间：
2012-12
期刊：
影响因子：
3.3
通讯作者：
Lakshmi Sundararajan;E. Lundquist
中科院分区：
文献类型：
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作者：
Lakshmi Sundararajan;E. Lundquist
神经元和神经嵴细胞的迁移对于神经系统的发育至关重要。在秀丽隐杆线虫中，左侧的QL成神经细胞向后迁移，右侧的QR向前迁移，尽管左右轴的谱系和出生位置相似。初始迁移是独立的Wnt信号，控制后来的前-后Q后裔迁移。先前的研究表明，跨膜蛋白<$A-40/DCC和<$A-21，一种新的含I型血小板反应蛋白重复序列的蛋白，在左侧QL后移中起冗余作用。在这里，我们表明，LAR受体蛋白酪氨酸磷酸酶PTP-3的行为与<$21平行<$40在QL后移。我们还表明，在右侧QR，的P40和PTP-3/P41 -21途径相互抑制对方的作用，在后方迁移，允许前QR迁移。最后，我们提出的证据表明，这些蛋白质的行为自主的Q成神经细胞。这些研究表明，Q神经母细胞在<$40、PTP-3和<$21功能方面存在固有的左右不对称性，这导致了向后迁移与向前迁移。
Migration of neurons and neural crest cells is of central importance to the development of nervous systems. In Caenorhabditis elegans, the QL neuroblast on the left migrates posteriorly, and QR on the right migrates anteriorly, despite similar lineages and birth positions with regard to the left–right axis. Initial migration is independent of a Wnt signal that controls later anterior–posterior Q descendant migration. Previous studies showed that the transmembrane proteins UNC-40/DCC and MIG-21, a novel thrombospondin type I repeat containing protein, act redundantly in left-side QL posterior migration. Here we show that the LAR receptor protein tyrosine phosphatase PTP-3 acts with MIG-21 in parallel to UNC-40 in QL posterior migration. We also show that in right-side QR, the UNC-40 and PTP-3/MIG-21 pathways mutually inhibit each other’s role in posterior migration, allowing anterior QR migration. Finally, we present evidence that these proteins act autonomously in the Q neuroblasts. These studies indicate an inherent left–right asymmetry in the Q neuroblasts with regard to UNC-40, PTP-3, and MIG-21 function that results in posterior vs. anterior migration.