Feikang granules ameliorate pulmonary inflammation in the rat model of chronic obstructive pulmonary disease via TLR2/4-mediated NF-κB pathway
Feikang granules ameliorate pulmonary inflammation in the rat model of chronic obstructive pulmonary disease via TLR2/4-mediated NF-κB pathway
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DOI：
10.1186/s12906-020-02964-x
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发表时间：
2020-06-03
影响因子：
3.9
通讯作者：
Wang, Yong
中科院分区：
文献类型：
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作者：
Yang, Liuliu;Wen, Minyong;Wang, Yong
目的：多篇报道显示中医药治疗慢性阻塞性肺疾病(COPD)可能是一种新的治疗方法，但其机制尚不清楚。本研究旨在观察肺康冲剂对慢性阻塞性肺疾病(COPD)模型大鼠的影响，并通过Toll样受体(TLR)/核因子-kappaB(NF-kappa B)信号通路探讨其可能的作用机制。结果：COPD模型大鼠肺组织中促炎细胞因子、IL-α、IL-1β、IL-6、IL-6和IL-17水平明显升高，肺组织中TLR4、TLR2、p-I kappa B、I kappa B和p65蛋白水平明显升高。TLR2和TLR4介导的核因子-kappaB信号通路在作用机制中发挥作用。肺康冲剂能显著降低内毒素诱导的肺组织和肺泡巨噬细胞炎性细胞因子的释放，并呈剂量依赖关系。这些中药还可抑制COPD大鼠肺组织中TLR2/4和I-kappa B的下调，并逆转肺组织中p-I-kappa B和NF-kappa B p65的上调。肺康冲剂对慢性阻塞性肺疾病大鼠的肺功能障碍和病理改变也有保护作用。结论：肺康冲剂具有预防肺部炎症、改善肺功能的作用，提示肺康冲剂可能是治疗慢性阻塞性肺疾病等慢性肺部疾病的有效药物。
Purpose: Several reports have shown that traditional Chinese medicine could be an alternative therapeutic approach for COPD patients, but the mechanism remains unknown. The present study aimed to examine the effects of Feikang granules in a COPD model rat and investigate the possible mechanisms via Toll-like receptor (TLR)/ nuclear factor kappa B (NF-kappa B) signaling.Methods: The COPD model rats were treated with Feikang granules, dexamethasone, or normal saline. The pulmonary function; lung tissue histology; levels of inflammatory cytokines; mRNA levels of TNF alpha, IL-6, TLR4, and TLR2; and protein levels of TLR4, TLR2, p-I kappa B, I kappa B and P65 in lung tissues were evaluated.Results: The present study confirmed that the pro-inflammatory cytokines, TNF-alpha, IL-1 beta, IL-6, and IL-17 levels were elevated and the pulmonary function and morphology are altered in COPD model rats. The TLR2 and TLR4-mediated NF-kappa B signaling pathway plays a role in the mechanism of action. Feikang granules, a type of Chinese herbal medicine, significantly reduced LPS induced inflammatory cytokines release from lung tissue and alveolar macrophage in a dose-dependent manner. These medical herbs also prevented TLR2/4 and I kappa B downregulation and reversed the p-I kappa B and NF-kappa B p65 upregulation of the lung tissue in the COPD rats. Feikang granules were also found to protect against pulmonary dysfunction and pathological changes in the COPD rats.Conclusion: The Chinese herbal medicine formula Feikang granules prevent pulmonary inflammation and improve pulmonary function, suggesting that Feikang granules may be an effective treatment for chronic pulmonary diseases, such as COPD.