Direct tissue analysis using matrix-assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation
Direct tissue analysis using matrix-assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation
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DOI：
10.1002/jms.505
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发表时间：
2003-07-01
影响因子：
2.3
通讯作者：
Caprioli, RM
中科院分区：
文献类型：
--
作者：
Schwartz, SA;Reyzer, ML;Caprioli, RM
提出了制备组织切片以通过基质辅助激光解吸/电离 (MALDI) 质谱直接分析的实用指南。描述了正确的样品处理技术，包括组织储存、切片和安装。重点放在优化基质参数，例如所用基质分子的类型、基质浓度和溶剂成分。说明了矩阵应用的几种不同技术。还讨论了最佳仪器参数以及直接组织分析方面先进数据分析方法的必要性。版权所有 (C) 2003 John Wiley Sons, Ltd.
Practical guidelines for the preparation of tissue sections for direct analysis by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry are presented. Techniques for proper sample handling including tissue storage, sectioning and mounting are described. Emphasis is placed on optimizing matrix parameters such as the type of matrix molecule used, matrix concentration, and solvent composition. Several different techniques for matrix application are illustrated. Optimal instrument parameters and the necessity for advanced data analysis approaches with regards to direct tissue analysis are also discussed. Copyright (C) 2003 John Wiley Sons, Ltd.