Peroxidase activity of enzymes bound to the ends of single-wall carbon nanotube forest electrodes
Peroxidase activity of enzymes bound to the ends of single-wall carbon nanotube forest electrodes
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DOI：
10.1016/s1388-2481(03)00076-6
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发表时间：
2003-05-01
影响因子：
5.4
通讯作者：
Rusling, JF
中科院分区：
文献类型：
--
作者：
Yu, X;Chattopadhyay, D;Rusling, JF
据我们所知，这是酶共价附着在垂直定向单壁碳纳米管（SWNT）森林阵列末端作为电极的第一个例子。铁血红素酶肌红蛋白和辣根过氧化物酶通过酰胺键偶联到纳米管森林的羧化末端，观察到准可逆的Fe-III/Fe-II伏安。结果表明，纳米管森林中的“树”在电行为上类似于金属，将电子从外部电路传导到酶的氧化还原位点。电化学表明，附着在SWNT森林上的肌红蛋白和辣根过氧化物酶具有过氧化物酶活性，过氧化氢在类似100 nM的缓冲溶液中具有检出限。这些原型swnt森林生物传感器很容易制备，酶层在几周内都是稳定的。(C) 2003 Elsevier Science B.V.版权所有
This communication reports the first example, to our knowledge, of enzymes covalently attached onto the ends of vertically oriented single-wall carbon nanotube (SWNT) forest arrays used as electrodes. Quasi-reversible Fe-III/Fe-II voltammetry was observed for the iron heme enzymes myoglobin and horseradish peroxidase coupled to carboxylated ends of the nanotube forests by amide linkages. Results suggest that the "trees" in the nanotube forest behaved electrically similar to a metal, conducting electrons from the external circuit to the redox sites of the enzymes. Electrochemically manifested peroxidase activity of myoglobin and horseradish peroxidase attached to the SWNT forests was demonstrated, with detection limits for hydrogen peroxide in buffer solutions of similar to100 nM. These prototype SWNT-forest biosensors are easy to prepare, and enzyme layers were stable for weeks. (C) 2003 Elsevier Science B.V. All rights reserved.