E1A TRANSCRIPTION INDUCTION - ENHANCED BINDING OF A FACTOR TO UPSTREAM PROMOTER SEQUENCES
E1A TRANSCRIPTION INDUCTION - ENHANCED BINDING OF A FACTOR TO UPSTREAM PROMOTER SEQUENCES
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DOI：
10.1126/science.2935935
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发表时间：
1986-02-14
期刊：
影响因子：
56.9
通讯作者：
NEVINS, JR
中科院分区：
文献类型：
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作者：
KOVESDI, I;REICHEL, R;NEVINS, JR
腺病毒E1A基因产物反式激活许多病毒和细胞启动子。这种转录诱导的机制进行了研究，在体内exoIII映射技术，以测定与E1A诱导型启动子相互作用的蛋白质。在野生型感染细胞中检测到与早期E2启动子结合的蛋白。在E1A诱导的情况下，在启动子的特异性相互作用不能被检测到，所示的exoIII保护片段的情况下。然而，如果建立了允许E2基因在不存在E1A的情况下转录的条件，则观察到与在存在E1A的情况下发现的相同的exoIII保护。这些结果表明，E2启动子的有效利用是由细胞转录因子介导的模型。在不存在E1A的情况下，可以发生相互作用，但与存在E1A的情况下的相互作用相比，缓慢且低效。
The adenovirus E1A gene product trans-activates a number of viral and cellular promoters. The mechanism for this transcriptional induction was investigated with an in vivo exoIII mapping technique to assay for proteins that interact with an E1A-inducible promoter. A protein bound to the early E2 promoter was detected in wild-type infected cells. In the absence of E1A induction, specific interactions at the promoter could not be detected, as indicated by the absence of an exoIII-protected fragment. However, if conditions were established that allowed transcription of the E2 gene in the absence of E1A, the same exoIII protection was observed as was found in the presence of E1A. These results suggest a model in which the efficient utilization of the E2 promoter is mediated by a cellular transcription factor. In the absence of E1A, the interaction can take place, but slowly and inefficiently in comparison with the interaction in the presence of E1A.