Beta-actin interacts with the E2 protein and is involved in the early replication of classical swine fever virus
Beta-actin interacts with the E2 protein and is involved in the early replication of classical swine fever virus
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DOI：
10.1016/j.virusres.2013.10.016
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发表时间：
2014-01-22
期刊：
影响因子：
5
通讯作者：
Qiu, Hua-Ji
中科院分区：
文献类型：
--
作者：
He, Fan;Ling, Lijun;Qiu, Hua-Ji
The E2 glycoprotein of classical swine fever virus (CSFV) is involved in viral infection and induction of neutralizing antibodies. Little is known about how E2 interacts with host cells. To understand the cellular factors involved in viral replication cycle, E2 was used as bait in yeast two-hybrid screens, resulting in the identification of beta-actin as a potential E2-interacting partner. E2-beta-actin interaction was confirmed by co-immunoprecipitation, GST pulldown, laser confocal and bimolecular fluorescence complementation assays. The E2-interacting domain of beta-actin was mapped to amino acids (aa) 95-188 and two beta-actin-interacting regions were identified in E2 (aa 182-261 and aa 262-341). Knockdown of beta-actin by RNA interference and disruption of filamentous beta-actin with cytochalasin D at 4 h post-infection caused a significant reduction of viral RNA copies and titers. Collectively, the results indicated that beta-actin is involved in the early replication of CSFV. (C) 2013 Elsevier B.V. All rights reserved.
