Choline acetyltransferase activity in rat heart after transplantation.
Choline acetyltransferase activity in rat heart after transplantation.
复制标题
移植后大鼠心脏中胆碱乙酰转移酶的活性。
DOI：
10.1152/ajpheart.1978.235.4.h367
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
R. Roskoski
中科院分区：
文献类型：
--
作者：
D. Lund;P. Schmid;S. E. Kelley;R. Corry;R. Roskoski
胆碱乙酰转移酶（Choline acetyltransferase， CAT）根据乙酰辅酶a +平衡的胆碱（Acetyl-CoA）的化学方程式催化生物合成乙酰胆碱。为了证明这种酶的神经元关系，我们测量了外源性神经支配被完全阻断的心脏的CAT活性，这是通过从供体动物中取出心脏并以异位位置移植到受体腹腔中来实现的。8天后处死动物，测量移植心脏的酶活性，并与受体心脏的酶活性进行比较。移植去神经心脏右心房胆碱乙酰转移酶活性下降98%，窦房结下降94%，左心房下降87%，右心室下降80%，室间隔和左心室下降约50%。这些数据表明，切除外源性副交感神经支配心脏导致CAT活性的可变降低，这一发现证实了CAT活性与外源性胆碱能神经支配有关。副交感神经系统的外源性（神经节前）成分是不均匀的，主要为心脏的特殊区域提供传导组织。残留的酶活性与内在（神经节后）副交感神经有关。内在成分均匀分布于心脏的特化区域和收缩区域。
Choline acetyltransferase (CAT) catalyzes the biosynthesis of acetylcholine according to the chemical equation: Acetyl-CoA + Choline in equilibrium Acetylcholine + CoA. To demonstrate the neuronal relationship of this enzyme, CAT activity was measured in hearts to which the extrinsic innervation was completely interrupted by extirpation of the heart from a donor animal and transplantation in a heterotopic position into the recipient's abdominal cavity. After the animals were killed at 8 days, enzyme activity in the transplanted hearts was measured and compared with that of the recipient. Choline acetyltransferase activity decreased 98% in the right atrium of the transplanted denervated hearts, 94% in the SA node, 87% in the left atrium, 80% in the right ventricle, and about 50% in the interventricular septum and left ventricle. These data show that sectioning the extrinsic parasympathetic innervation to the heart results in variable decreases in CAT activity, a finding which confirms that CAT activity is related to the extrinsic cholinergic innervation. The extrinsic (preganglionic) component of the parasympathetic system is nonuniform and supplies predominantly the specialized regions of the heart with conduction tissue. The residual enzyme activity is related to intrinsic (postganglionic) parasympathetic neurons. The intrinsic component is uniformly distributed to both specialized and contractile regions of the heart.