INDUCTION OF HEPATITIS-B SURFACE-ANTIGEN IN HUMAN HEPATOMA-DERIVED CELL-LINES
INDUCTION OF HEPATITIS-B SURFACE-ANTIGEN IN HUMAN HEPATOMA-DERIVED CELL-LINES
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DOI：
10.1099/0022-1317-53-1-105
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发表时间：
1981-01-01
影响因子：
3.8
通讯作者：
DREESMAN, GR
中科院分区：
文献类型：
--
作者：
OEFINGER, PE;BRONSON, DL;DREESMAN, GR
研究了两株人肝癌细胞株乙型肝炎病毒表面抗原（HBsAg）的表达。PLC/PRF/5细胞连续向培养基中分泌HBsAg，而Mahlavu细胞不分泌抗原。然而，在Mahlavu细胞中检测到细胞质抗原的比例很低（<5%）。用地塞米松（DXM）、5-碘-2′-脱氧尿苷（IdUrd）或两者处理的培养物也检测HBsAg的表达。结果表明：(1)DXM刺激PLC/PRF/5细胞分泌HBsAg，而对Mahlavu细胞没有刺激作用；（ii）如果每隔24至48小时更换培养基，在任何培养中，表达细胞质HBsAg的Mahlavu细胞的百分比都没有增加，但增加了大约。在不改变培养基的情况下，用DXM或IdUrd/DXM处理的培养在4天内增加5倍。然而，在任何DXM培养中，表达细胞质抗原的PLC/PRF/5细胞的免疫过氧化物酶染色强度未见增加；（iii）与DXM培养相比，IdUrd/DXM培养对HBsAg表达的刺激程度较低，而IdUrd培养对HBsAg表达的刺激程度不高。因此，DXM在24 h内增强PLC/PRF/5细胞分泌HBsAg，并在延迟后增强Mahlavu细胞胞质抗原的表达。而Mahlavu细胞的抗原分泌明显受阻。
Cultures of two human hepatoma cell lines were examined for expression of hepatitis B virus surface antigen (HBsAg). The PLC/PRF/5 cells secreted HBsAg continuously into the culture medium, whereas Mahlavu cells did not secrete the antigen. However, cytoplasmic antigen was detected in a low percentage (<5%) of the Mahlavu cells. The expression of HBsAg also was assayed in cultures treated with dexamethasone (DXM), 5-iodo-2′-deoxyuridine (IdUrd), or both. The results demonstrated that: (i) DXM stimulated secretion of HBsAg by PLC/PRF/5 cells but not by Mahlavu cells; (ii) the percentage of Mahlavu cells expressing cytoplasmic HBsAg was not increased in any cultures if the medium was replaced at 24 to 48 h intervals but was increased approx. fivefold within 4 days in cultures treated with DXM or IdUrd/DXM if the medium was not changed. However, no increase was noted in the intensity of the immunoperoxidase stain of PLC/PRF/5 cells that expressed cytoplasmic antigen in any DXM cultures; (iii) HBsAg expression was stimulated to a lesser extent in IdUrd/DXM cultures than in DXM cultures and was not enhanced in IdUrd cultures. Thus, DXM enhanced secretion of HBsAg by PLC/PRF/5 cells within 24 h and, after a delay, enhanced expression of cytoplasmic antigen by Mahlavu cells. However, antigen secretion by Mahlavu cells evidently was blocked.