MicroRNA-21 (miR-21) represses tumor suppressor PTEN and promotes growth and invasion in non-small cell lung cancer (NSCLC)
MicroRNA-21 (miR-21) represses tumor suppressor PTEN and promotes growth and invasion in non-small cell lung cancer (NSCLC)
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DOI：
10.1016/j.cca.2010.02.074
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发表时间：
2010-06-03
影响因子：
5
通讯作者：
Yang, Guang-hai
中科院分区：
文献类型：
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作者：
Zhang, Ji-guang;Wang, Jian-jun;Yang, Guang-hai
背景资料：microRNAs（miRNAs）是一类调控基因表达的小分子非编码RNA，在人类疾病（包括恶性肿瘤）的发病机制中发挥重要作用。miR-21是一种在多种类型恶性肿瘤中普遍过表达的miRNA，可能通过靶向肿瘤抑制基因影响肿瘤进展。方法：采用qRT-PCR和western blot方法分别检测20例配对非小细胞肺癌（NSCLC）组织和癌旁正常肺组织中miR-21和PTEN的表达。在具有miR-21抑制剂以降低miR-21表达的NSCLC细胞系中评估miR-21对PTEN表达的影响。结果：与癌旁组织相比，miR-21在肿瘤组织中高表达。值得注意的是，具有晚期临床TNM分期（n = 16）或远端转移（n = 5）的患者比没有这些患者（n = 26或n = 37）表现出更高的miR-21表达（p
Background: MicroRNAs (miRNAs) are a class of small non-coding RNAs regulating gene expression that play roles in the pathogenesis of human diseases, including malignancy. miR-21, a commonly overexpressed miRNA in very diverse types of malignancies, may affect tumor progression through targeting tumor suppressor genes. We identified the role of miR-21 in non-small cell lung cancer (NSCLC) and to clarify the regulation of PTEN by miR-21 and determine mechanisms of this regulation.Methods: Expression of miR-21 and PTEN in 20 paired NSCLC and adjacent non-tumor lung tissues was investigated by qRT-PCR and western blot, respectively. The effect of miR-21 on PTEN expression was assessed in NSCLC cell lines with miR-21 inhibitor to decrease miR-21 expression. Furthermore, the roles of miR-21 in cell growth and invasion were analyzed with miR-21 inhibitor-transfected cells.Results: miR-21 was overexpressed in tumor tissues relative to adjacent non-tumor tissues. Notably, patients with advanced clinical TNM stage (n = 16) or distal metastasis (n = 5) demonstrated higher miR-21 expression than those without them (n = 26, or n = 37) (p