Neuroprotective effects of almond skins in experimental spinal cord injury
Neuroprotective effects of almond skins in experimental spinal cord injury
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DOI：
10.1016/j.clnu.2010.08.002
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发表时间：
2011-04-01
影响因子：
6.3
通讯作者：
Cuzzocrea, S.
中科院分区：
文献类型：
--
作者：
Mandalari, G.;Genovese, T.;Cuzzocrea, S.
背景与目的：脊髓损伤后的功能缺陷既有机械性损伤，也有继发性组织炎症反应。用天然杏仁皮(NS)制作脊髓损伤动物模型，观察其抗炎作用。方法：采用四节段T5-T8椎板切除的方法，在硬脑膜上夹闭血管夹造成脊髓损伤。在本研究中，为了阐明生理盐水的保护作用是否与总酚含量有关，我们还研究了漂白(BS)杏仁皮(工业上通过从坚果中去除麸皮获得)在脊髓损伤中的作用。分别于SCI后1h、6h灌胃生理盐水和BS(30 mg/kg)。结果：SCI小鼠脊髓损伤严重，表现为水肿、组织损伤、炎性介质产生和细胞凋亡(Bax、Bcl2和TUNEL法检测)。生理盐水治疗组脊髓损伤后1h和6h，中性粒细胞浸润、核因子-kappaB活化、PAR形成、iNOS表达和细胞凋亡等炎症指标均明显降低。结论：生理盐水治疗可减少炎症反应和组织损伤，对脊髓损伤有一定的治疗作用。(C)2010年爱思唯尔有限公司和欧洲临床营养与代谢学会。版权所有。
Background & Aims: Functional deficits following spinal cord injury (SCI) arise from both mechanical injury and from secondary tissue reactions involving inflammation. Natural almond skins (NS) were tested to evaluate anti-inflammatory effects on an animal model of SO.Methods: SCI was induced by the application of vascular clips to the dura via a four-level T5-T8 laminectomy. In the present study, to elucidate whether the protective effects of NS are related to the total phenolic content, we also investigated the effect of a blanched (BS) almond skins (industrially obtained by removing bran from the nut) in SCI. NS and BS (30 mg/kg respectively) were administered per os, 1 h and 6 h, after SCI.Results: SCI in mice resulted in severe injury characterized by edema, tissue damage, production of inflammatory mediators and apoptosis (measured by Bax, Bcl-2 and Tunel assay). NS treatment, 1 and 6 h after SCI, reduced all parameters of inflammation as neutrophil infiltration, NF-kappa B activation, PAR formation, iNOS expression and apoptosis. However, treatment with BS did not exert any protective effect.Conclusions: Our results suggest that NS treatment, reducing the development of inflammation and tissue injury, may be useful in the treatment of SCI. (C) 2010 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.