Imaging thrombus with radiolabelled monoclonal antibody to platelets.
Imaging thrombus with radiolabelled monoclonal antibody to platelets.
复制标题
使用放射性标记的血小板单克隆抗体对血栓进行成像。
作者：
A. Peters;J. Lavender;S G Needham;I. Loutfi;D. Snook;A. Epenetos;P. Lumley;R. Keery;N. Hogg
铟-111-羟基喹啉标记的血小板虽然可用于检测血栓，但由于其制备所需的时间和技术技能而没有得到广泛使用。因此，进行了一项研究，以评价一种新的方法成像血栓与血小板放射性标记的111 In标记的单克隆抗体，P256，针对血小板表面糖蛋白复合物IIb/IIIa。当P256所占据的受体数量小于血小板表面上可用的总受体的3%时，通过血小板聚集测定法评估的血小板功能未受干扰。使用二亚乙基三胺五乙酸用111 In放射性标记P256，其达到185 MBq（5 mCi）/mg的比活度。在该特异性活性下未检测到免疫反应性受损。血小板标记的放射性标记的单克隆抗体在体外的两名患者的受体占有率为6%，在体内-即，通过直接静脉注射P256-在6名患者的受体占有率为1%。体内回收和生物分布动力学表明，在体外标记后，血小板被最低限度地激活。静脉注射P256后记录的111 In动力学表明血小板快速有效的放射性标记，没有血小板活化的迹象。在接受静脉注射P256的6名患者中，3名患者有血栓记录，其中2名患者的P256血小板造影结果呈阳性。第3例受试者患有慢性深静脉血栓形成，血栓造影阴性。使用针对血小板的放射性标记的单克隆抗体对血栓进行成像似乎作为一种简单的、非侵入性的血栓诊断方法提供了巨大的潜力。
Indium-111-hydroxyquinoline labelled platelets, though useful in the detection of thrombus, have not gained widespread use owing to the time and technical skill required for their preparation. A study was therefore conducted evaluating a new method of imaging thrombus with platelets radiolabelled with a 111In labelled monoclonal antibody, P256, directed to the platelet surface glycoprotein complex IIb/IIIa. When the number of receptors occupied by P256 was less than 3% of the total available on the platelet surface platelet function, as assessed by platelet aggregometry, was undisturbed. P256 was radiolabelled with 111In using diethylenetriaminepenta-acetic acid, which achieved a specific activity of 185 MBq (5 mCi)/mg. No impairment of immunoreactivity was detected at this specific activity. Platelets were labelled with radiolabelled monoclonal antibody in vitro in two patients at a receptor occupancy of 6% and in vivo--that is, by direct intravenous injection of P256--in six patients at a receptor occupancy of 1%. In vivo recovery and biodistribution kinetics suggested that after in vitro labelling platelets were minimally activated. The 111In kinetics recorded after intravenous P256 suggested rapid and efficient radiolabelling of platelets and gave no indication of platelet activation. Of the six patients who received intravenous P256, three had documented thrombus, two of whom gave positive results on P256 platelet scintigraphy. The third subject had chronic deep venous thrombosis and was scintigraphically negative. Imaging thrombus using a radiolabelled monoclonal antibody directed to platelets appears to offer great potential as a simple, non-invasive approach to the diagnosis of thrombosis.
DOI：
10.1073/pnas.81.21.6851
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MORRISON, SL;JOHNSON, MJ;OI, VT
通讯作者：
OI, VT
DOI：
10.1172/jci109983
发表时间：
1980-12
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
R. McEver;N. Baenziger;P. Majerus
通讯作者：
R. McEver;N. Baenziger;P. Majerus