Amino acid membrane transport properties of L1210 cells resistant to cisplatin.
Amino acid membrane transport properties of L1210 cells resistant to cisplatin.
复制标题
顺铂耐药的 L1210 细胞的氨基酸膜转运特性。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
Scanlon Kj
中科院分区：
文献类型：
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作者：
Grossman Rb;Scanlon Kj
使小鼠L1210白血病细胞系对化疗剂顺铂具有18倍抗性。耐药细胞的形态和生化特性与敏感细胞不同。在耐药细胞中，标记顺铂的摄取仅略有下降。然而，这些细胞的外源性甲硫氨酸的需求是18倍低于亲本细胞。甲硫氨酸需求的减少也与甲硫氨酸代谢和转运的差异有关。膜转运分析显示，不同的Kt和Vmax特性的蛋氨酸和nonmetabolizable氨基酸类似物之间的两个细胞系。在对顺铂耐药的L1210细胞中，氨基酸转运减少，加上氨基酸底物特异性的变化，可能与药物膜现象有关，这可能是对化疗药物耐药的一种机制。
A mouse L1210 leukemia cell line was made 18-fold resistant to the chemotherapeutic agent cisplatin. The drug resistant cells exhibited changes in morphologic and biochemical properties from the sensitive cells. Uptake of labeled cisplatin was only slightly decreased in the resistant cells. However, the exogenous methionine requirement for these cells was 18-fold less than for parent cells. This decrease in the methionine requirement was also associated with differences in both methionine metabolism and transport. Membrane transport analysis revealed different Kt and Vmax properties for methionine and a nonmetabolizable amino acid analogue between the two cell lines. In L1210 cells resistant to cisplatin, decreases in amino acid transport, plus changes in amino acid substrate specificities, may be related to a drug-membrane phenomenon which could be one mechanism of resistance to chemotherapeutic agents.