Cloning and Characterisation of Novel Single Locus Probes for Forensic Purposes
Cloning and Characterisation of Novel Single Locus Probes for Forensic Purposes
复制标题
用于法医目的的新型单基因座探针的克隆和表征
DOI：
10.1007/978-3-642-77324-2_37
复制
发表时间：
1992
影响因子：
3
通讯作者：
K. Sullivan
中科院分区：
文献类型：
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作者：
C. Kimpton;R. Hopgood;S. Watson;P. Gill;K. Sullivan
通过对多基因座探针的分析(Jeffreys等人，1986；Fowler等人，1988)，先前已经证明在人类基因组中存在大量的高变量基因座。许多高变基因座已被偶然发现(Wyman and White，1980；Bell et al，1982；Higgs et al，1981；Capon et al，1983)。Wong等人(1986,1987)使用人类DNA的lambda文库，开发了一种专门用于分离高变量DNA的克隆策略；插入片段由大小选定的高分子量(5-15kB)DNA片段组成。这一过程丰富了重复的小卫星文库。最近，Armour等人(1990)提出了一种更成功、更简单的方法，通过构建Charomid文库来克隆高变量DNA(Saito和Stark，1986)。Charomid是粘粒衍生的载体，没有lambda载体的相同大小限制，因此非常不稳定的小卫星片段的克隆有很大的改进，这些小卫星片段往往会丢失重复。
From the analysis of multi-locus probes (Jeffreys et al, 1986; Fowler et al, 1988) it has previously been demonstrated that there are numerous hypervariable loci in the human genome. Many hypervariable loci have been fortuitously identified (Wyman and White, 1980; Bell et al, 1982; Higgs et al, 1981; Capon et al, 1983). A cloning strategy, used specifically to isolate hypervariable DNA was developed by Wong et al (1986, 1987) who used lambda libraries of human DNA; the insert consisting of size selected high molecular weight (5–15kB) DNA fragments. This procedure enriched the library for tandemly repeated minisatellites. Recently, Armour et al (1990) introduced a more successful and simpler method for cloning hypervariable DNA by constructing Charomid libraries (Saito and Stark, 1986). Charomids are cosmid derived vectors which do not have the same size constraints of lambda vectors, hence the cloning of rather unstable minisatellite fragments, which tend to lose repeats, is much improved.
影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs