Zebrafish: a new companion for translational research in oncology.
Zebrafish: a new companion for translational research in oncology.
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DOI：
10.1158/1078-0432.ccr-14-2921
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发表时间：
2015-03-01
期刊：
影响因子：
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通讯作者：
Hurlstone A
中科院分区：
文献类型：
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作者：
Barriuso J;Nagaraju R;Hurlstone A
在高通量“组学”技术的时代，可以生成的前所未有的数据量为试图提供个性化治疗的肿瘤学家提供了重大机遇，但同时也是一个更大的挑战。传统上，新靶点的临床前测试和针对这些靶点的活性化合物的鉴定需要广泛使用已建立的人类细胞系以及遗传修饰的小鼠肿瘤模型。斑马鱼中患者来源的异种移植物可能在不久的将来为选择适当的个性化治疗提供平台，并与斑马鱼转基因肿瘤模型一起代表药物开发的替代载体。斑马鱼很容易被基因改造。斑马鱼胚胎的透明性和最近开发的色素缺陷斑马鱼为研究人员提供了直接观察活体脊椎动物宿主中癌症形成和进展的宝贵能力。斑马鱼适合移植试验，该试验测试荧光标记的肿瘤细胞的连续传代以及它们的扩散和/或转移能力。迄今为止在基因工程和异种移植方面取得的进展将使斑马鱼成为癌症研究中最通用的动物模型之一。可用于转基因、移植试验、单细胞功能试验和体内成像研究的模式生物使斑马鱼成为小鼠在转化肿瘤学研究中的天然伴侣。
In an era of high-throughput ‘omic’ technologies, the unprecedented amount of data that can be generated presents a significant opportunity but simultaneously an even greater challenge for oncologists trying to provide personalized treatment. Classically, pre-clinical testing of new targets and identification of active compounds against those targets has entailed the extensive use of established human cell lines, as well as genetically modified mouse tumor models. Patient-derived xenografts in zebrafish may in the near future provide a platform for selecting an appropriate personalized therapy and together with zebrafish transgenic tumor models represent an alternative vehicle for drug development. The zebrafish is readily genetically modified. The transparency of zebrafish embryos and the recent development of pigment deficient zebrafish afford researchers the valuable capacity to observe directly cancer formation and progression in a live vertebrate host. The zebrafish is amenable to transplantation assays that test the serial passage of fluorescently labelled tumor cells as well as their capacity to disseminate and/or metastasize. Progress achieved to date in genetic engineering and xenotransplantation will establish the zebrafish as one of the most versatile animal models for cancer research. A model organism that can be used in transgenesis, transplantation assays, single cell functional assays and in vivo imaging studies make zebrafish a natural companion for mice in translational oncology research.