Activation of p44/p42 mitogen-activated protein kinase limits triiodothyronine-stimulated alkaline phosphatase activity in osteoblasts
Activation of p44/p42 mitogen-activated protein kinase limits triiodothyronine-stimulated alkaline phosphatase activity in osteoblasts
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DOI：
10.1006/bbrc.2001.5515
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发表时间：
2001-09-07
影响因子：
3.1
通讯作者：
Uematsu, T
中科院分区：
文献类型：
--
作者：
Kozawa, O;Hatakeyama, D;Uematsu, T
研究表明，甲状腺激素刺激成骨细胞中碱性磷酸酶的活性，碱性磷酸酶是成熟成骨细胞表型的标志物。在本研究中，我们研究了p44/p42丝裂原活化蛋白是否。(MAP)激酶参与成骨样MC 3 T3-E1细胞中甲状腺素刺激的碱性磷酸酶活性。三碘甲腺原氨酸（T-3）可明显诱导p44/p42 MAP激酶磷酸化。PD 98059和U 0126是激活p44/p42 MAP激酶的上游激酶的抑制剂，以剂量依赖性方式显著增强T-3诱导的碱性磷酸酶活性。U 0126可降低T-3诱导的p44/p42 MAP激酶磷酸化。提示p44/p42 MAP激酶参与甲状腺激素刺激的碱性磷酸酶活性。p44/p42 MAP激酶在甲状腺激素的抑制作用中起重要作用。(C)北京：科学出版社.
It has been shown that thyroid hormone stimulates the activity of alkaline phosphatase, a marker of mature osteoblast phenotype, in osteoblasts. In the present study, we investigated whether p44/p42 mitogen-activated protein. (MAP) kinase is involved in the thyroid hormone-stimulated alkaline phosphatase activity in osteoblast-like MC3T3-E1 cells. Triiodothyronine (T-3) markedly induced the phosphorylation of p44/p42 MAP kinase. PD98059 and U0126, inhibitors of the upstream kinase that activates p44/p42 MAP kinase, significantly enhanced the T-3-induced alkaline phosphatase activity in a dose-dependent manner. The phosphorylation of p44/p42 MAP kinase induced by T-3 was reduced by U0126. These results strongly suggest that p44/p42 MAP kinase takes part in the thyroid hormone-stimulated alkaline phosphatase activity in. osteoblasts and that p44/p42 MAP kinase plays an inhibitory role in the thyroid hormone-effect. (C) 2001 Academic Press.