Recovery of labeled CO2 during the infusion of C-1- vs C-2-labeled acetate: implications for tracer studies of substrate oxidation.
Recovery of labeled CO2 during the infusion of C-1- vs C-2-labeled acetate: implications for tracer studies of substrate oxidation.
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DOI：
10.1093/ajcn/51.2.248
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发表时间：
1990-02
期刊：
影响因子：
--
通讯作者：
R R Wolfe-R;F. Jahoor
中科院分区：
文献类型：
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作者：
R R Wolfe-R;F. Jahoor
在这项研究中，我们确定了正常人类志愿者和麻醉狗体内碳标记的乙酸盐转化为二氧化碳的速率。在人类受试者（n = 4）中，[1-13C]乙酸盐被输注一次，并且在同一受试者的重复研究中输注[2-13C]乙酸盐。在吸收后志愿者 (n = 6) 中，当输注 [1-13C]- 或 [2-13C] 乙酸盐时，分别有 81.2 +/- 6.5%（平均值 +/- SEM）和 53.1 +/- 7.4% 的 13C 被回收为 13CO2。在一名研究对象中，在 3.5 mg.kg-1.min-1 的外源性葡萄糖输注过程中，从 [1-13C]- 和 [2-13C] 乙酸盐中回收 13CO2 分别为 72.7% 和 38.5%。在狗中，同时输注[1-14C]-和[2-13C]乙酸盐。 14CO2 的回收率为注入同位素的 75.9 +/- 2.5%，而 13CO2 的回收率为 40.8 +/- 1.9%。我们得出的结论是，乙酰辅酶 A (CoA) 中的标记位置决定了标记的乙酰辅酶 A 的氧化过程在标记的二氧化碳排泄中反映的程度。
In this study we determined the rate of conversion of carbon-labeled acetate to carbon dioxide in normal human volunteers and in anesthetized dogs. In human subjects (n = 4), [1-13C]acetate was infused on one occasion, and [2-13C]acetate was infused in the repeat study in the same subjects. In postabsorptive volunteers (n = 6), 81.2 +/- 6.5% (mean +/- SEM) and 53.1 +/- 7.4% of infused 13C was recovered as 13CO2 when [1-13C]- or [2-13C]acetate, respectively, were infused. In one subject studied during exogenous glucose infusion at 3.5 mg.kg-1.min-1, recovery of 13CO2 was 72.7% and 38.5% from [1-13C]- and [2-13C]acetate, respectively. In dogs, [1-14C]- and [2-13C]acetate were infused simultaneously. Recovery of 14CO2 was 75.9 +/- 2.5% of infused isotope whereas recovery of 13CO2 was 40.8 +/- 1.9%. We concluded that the position of the label in acetyl coenzyme A (CoA) determines the extent to which the process of oxidation of labeled acetyl CoA is reflected in labeled carbon dioxide excretion.