Occurrence in Saccharomyces cerevisiae of a gene homologous to the cDNA coding for the alpha subunit of mammalian G proteins.
Occurrence in Saccharomyces cerevisiae of a gene homologous to the cDNA coding for the alpha subunit of mammalian G proteins.
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酿酒酵母中存在与编码哺乳动物 G 蛋白 α 亚基的 cDNA 同源的基因。
DOI：
10.1073/pnas.84.8.2140
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发表时间：
1987
影响因子：
11.1
通讯作者：
Y. Kaziro
中科院分区：
文献类型：
--
作者：
M. Nakafuku;H. Itoh;S. Nakamura;Y. Kaziro
从交叉杂交研究与cDNAs的大鼠脑鸟嘌呤核苷酸结合调节（G）蛋白的α亚基的代码，我们已经从酵母酿酒酵母编码的氨基酸序列是高度同源的G蛋白，介导抑制腺苷酸环化酶（Gi α）从大鼠脑的α亚基的基因。该基因暂时命名为GPA 1，包含一个连续的，单一的开放阅读框架的1416个核苷酸，编码472个氨基酸的蛋白质，计算的Mr为54，075。预测的由GPA 1基因编码的蛋白质的氨基酸序列（暂时命名为G蛋白1 α或GP 1 α）与大鼠脑Gi α和功能未知的G蛋白的α亚基（Go α）的氨基酸序列显著同源; GTP水解位点的一级结构以及GTP相互作用几乎相同。酵母GP 1 α（472个氨基酸）和大鼠脑Gi α（355个氨基酸）分子大小的主要差异是由于酵母GP 1 α中存在110个额外氨基酸残基的延伸，其插入哺乳动物Gi α的NH 2末端三分之一附近。从印迹杂交分析，GP 1 α mRNA的大小估计为1.7个酶。
From cross-hybridization studies with cDNAs that code for the alpha subunits of rat brain guanine nucleotide-binding regulatory (G) proteins, we have isolated a gene from yeast Saccharomyces cerevisiae encoding an amino acid sequence that is highly homologous to the alpha subunit of the G protein that mediates inhibition of adenylate cyclase (Gi alpha) from rat brain. The gene, tentatively designated as GPA1, contains a contiguous, single open reading frame of 1416 nucleotides that codes for a protein of 472 amino acids with a calculated Mr of 54,075. The predicted amino acid sequence of the protein encoded by the GPA1 gene (tentatively designated as G protein 1 alpha or GP1 alpha) is remarkably homologous to the amino acid sequence of rat brain Gi alpha and the alpha subunit of the G protein of unknown function (Go alpha); the primary structure of the sites for GTP hydrolysis as well as GTP interaction are nearly identical. The main difference in the molecular sizes of yeast GP1 alpha (472 amino acids) and rat brain Gi alpha (355 amino acids) is due to the presence of a stretch of 110 extra amino acid residues in yeast GP1 alpha, which are inserted near the NH2-terminal one-third of mammalian Gi alpha. From blot-hybridization analysis, the size of the GP1 alpha mRNA was estimated as 1.7 kilobases.