Escherichia coli ribosome is inactivated by Mirabilis antiviral protein which cleaves the N-glycosidic bond at A2660 of 23 S ribosomal RNA.
Escherichia coli ribosome is inactivated by Mirabilis antiviral protein which cleaves the N-glycosidic bond at A2660 of 23 S ribosomal RNA.
复制标题
紫茉莉抗病毒蛋白可裂解 23 S 核糖体 RNA A2660 处的 N-糖苷键，使大肠杆菌核糖体失活。
DOI：
10.1016/0022-2836(91)80168-t
复制
发表时间：
1991
影响因子：
5.6
通讯作者：
M. Noma
中科院分区：
文献类型：
--
作者：
N. Habuka;M. Miyano;J. Kataoka;M. Noma
已知核糖体失活蛋白（RIPs）能使真核核糖体失活，从而导致蛋白质合成的抑制，但没有证据表明它们能使大肠杆菌的核糖体失活。近年来，Mirabilis抗病毒蛋白（Mirabilis antiviral protein， MAP），一种RIP，被证实可以抑制e蛋白的合成。以及真核生物。为了阐明其机理，E。采用聚丙烯酰胺/琼脂糖复合凝胶电泳和DNA引物逆转录酶RNA测序对MAP处理的核糖体进行分析。23个S rrna，核糖体的a260值为15，在100 nmat 37°C下用MAP处理30分钟，然后用苯胺处理，在体外完全切割。而在相同条件下，蓖麻毒素链对其没有影响。在RNA测序中，DNA聚合的引物延伸在23s rRNA的A2660之前停止。此外，MAP和苯胺处理均可切割16 S和23 S rnas。用colirRNAs作为底物，在RNA测序中引物延伸分别在碱基A2660和A1014之前停止。由于A2660区域已被证明与延伸因子EF-Tu和EF-G相互作用，这些结果表明MAP在A2660 inE上切割了n -糖苷键。导致核糖体失活的大肠杆菌23s RNA。
Ribosome-inactivating proteins (RIPs) are known to inactivate eukaryotic ribosomes, which results in the inhibition of protein synthesis, but there has been no evidence that they inactivate the ribosomes ofEscherichia coli. Recently, Mirabilis antiviral protein (MAP), a RIP, has been shown to inhibit the protein synthesis ofE. colias well as eukaryotes. To elucidate its mechanism,E. coliribosomes treated with MAP were analyzed by polyacrylamide/agarose composite gel electrophoresis and RNA sequencing using reverse transcriptase with DNA primer. The 23 S rRNAs, with an A260value for ribosomes of 15, were completely cleavedin vitroby a 30 minute treatment with MAP at a concentration of 100 nmat 37°C and a subsequent treatment with aniline. However, they were not affected by ricinA-chain under the same conditions. The primer extension of DNA polymerization stopped before A2660 of 23 S rRNA in RNA sequencing. Furthermore, both 16 S and 23 S rRNAs were cleaved by the MAP and aniline treatments when nakedE. colirRNAs were used as substrates, and the primer extension stopped before bases A2660 and A1014, respectively, in RNA sequencing. As the A2660 region has been shown to interact with the elongation factors EF-Tu and EF-G these results indicate that MAP cleaves theN-glycosidic bond at A2660 inE. coli23 S RNA resulting in the inactivation of the ribosome.
DOI：
10.1016/s0021-9258(18)34241-8
发表时间：
1982-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Y. Endo;I. Wool
通讯作者：
Y. Endo;I. Wool
大鼠 28S 核糖体核糖核酸中 α-sarcin 切割位点的核苷酸序列。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chan,YL;Endo,Y;Wool,IG
通讯作者：
Wool,IG