MORPHOGENESIS AND METABOLISM OF AMPHIBIAN LARVAE AFTER EXCISION OF HEART .1. MORPHOGENESIS OF HEARTLESS TADPOLES OF RANA PIPIENS
MORPHOGENESIS AND METABOLISM OF AMPHIBIAN LARVAE AFTER EXCISION OF HEART .1. MORPHOGENESIS OF HEARTLESS TADPOLES OF RANA PIPIENS
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DOI：
10.1002/ar.1091170306
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发表时间：
1953-01-01
影响因子：
--
通讯作者：
KEMP, NE
中科院分区：
文献类型：
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作者：
KEMP, NE
在沙姆韦阶段22或23切除心脏后，淡色隐翅虫的幼虫存活可达6天(通常为4或5天)。由此产生的严重异常包括：由于肿胀而导致的头部增宽；小头畸形；眼睛静止和缩小；扩张的真皮黑素载体分离，随后在肿大的淋巴空间上出现较浅的外观；前肾周围肿胀和背侧肠系膜膨胀；消化道无法延长；对触觉刺激的反应减弱。显微镜下可辨别的异常有：眼后房塌陷；头和体壁淋巴空间增大；前肾小管、隆突后窦和背主动脉增大；背侧肠系膜分裂成两片叶子，因积液而广泛分开。外周血中仍有血细胞，其中一些处于有丝分裂状态。动物于术后5天固定。在存活期间，消化道上皮细胞经历了广泛的分化，大量的蛋黄被消化，但这些过程比对照标本进行得慢得多。这些异常与其他工作人员在两栖类幼虫暴露于X射线、N芥子和各种其他药物后获得的异常之间的相似性进行了讨论。
Larvae of R. pipiens live up to 6 days (usually 4 or 5 days) after excision of the heart at Shumway stage 22 or 23. Resultant gross anomalies include: broadening of the head due to swelling; microcephaly; immobility and reduced size of eyes; separation of expanded dermal melanophores and consequent lighter appearance over swollen lymph spaces; swelling around the pronephroi and ballooning of the dorsal mesentery; failure of the digestive tract to elongate; and reduced response to tactile stimuli. Microscopically discernible anomalies are: collapse of the posterior chamber of the eye; enlargement of lymph spaces in the head and body wall; enlargement of pronephric tubules, postcardinal sinuses, and dorsal aorta; splitting of the dorsal mesentery into 2 leaves widely separated by accumulation of fluid. Blood cells, some in mitosis, were still present in an exptl. animal fixed 5 days after operation. Epithelia of the digestive tract undergo extensive differentiation and considerable yolk is digested during the period of survival, but these processes proceed much more slowly than in control specimens. Similarities between these anomalies and those obtained by other workers after exposure of amphibian larvae to X-rays, N mustards and a variety of other drugs are discussed.