TESTICULAR INTERSTITIAL FLUID AS A MONITOR FOR CHANGES IN THE INTRATESTICULAR ENVIRONMENT IN THE RAT
TESTICULAR INTERSTITIAL FLUID AS A MONITOR FOR CHANGES IN THE INTRATESTICULAR ENVIRONMENT IN THE RAT
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DOI：
10.1530/jrf.0.0690125
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
COOPER, I
中科院分区：
文献类型：
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作者：
SHARPE, RM;COOPER, I
使用从大鼠睾丸收集的睾丸间质液（IF）被证实是睾丸细胞外管外液总量的指标（反映睾丸毛细血管的渗透性），也是测量间质激素环境变化的一种手段。前者在注射hCG（人绒毛膜促性腺激素）后0-40 h，通过比较对照组和双侧隐睾大鼠的白蛋白间隙与相同试验中回收的IF体积进行测试。虽然恢复的IF体积平均仅为白蛋白空间的50%，但注射hCG后，这两项指标并行增加，并且在4至5倍的范围内始终密切相关（P < 0.001）。睾酮恢复量随年龄的增加而增加，与睾丸重量的增加平行，睾酮浓度与外周血清的变化平行，从45 ~ 80日龄先升高后下降。注射后25 .亩。g牛黄体生成素，IF、精浆静脉（SV）和外周静脉（PV）血液中的睾酮水平在1小时内增加了10倍，并在接下来的11小时内恢复到对照水平。IF血液中的睾酮水平总是比SV血液中的睾酮水平高得多，但这种差异并不是恒定的。注射LHRH激动剂的大鼠sc导致血清LH水平以及睾酮的IF和PV水平平行增加。然而，在6小时时，睾酮水平出现了与LH无关的二次增加，这与IF体积的增加有关，反映了毛细血管壁通透性的增加，从而增加了LH进入睾丸的运输。
The use of testicular interstitial fluid (IF) collected from the rat testis was validated as an index of the total extracellular extratubular fluid volume of the testis (which reflects the permeability of testicular capillaries) and as a means of measuring changes in the interstitial hormonal environment. The former was tested by comparing the albumin space with the volume of recovered IF in the same tests from control and bilaterally cryptorchid rats sampled at 0-40 h after injection of hCG [human chorionic gonadotropin]. Although the volume of IF recovered was on average only 50% of the albumin space, both measures increased in parallel after hCG injection and were always closely correlated (P < 0.001) over a 4- to 5-fold range. The volume of recovered IF increased with age in parallel with increase in testicular wt, and the testosterone concentration in IF paralleled changes in peripheral serum, increasing from 45 to 80 days of age and then declining. After injection of 25 .mu.g bovine LH [luteinizing hormone], testosterone levels in IF, spermatic venous (SV) and peripheral venous (PV) blood increased up to 10-fold by 1 h and returned to control levels over the next 11h. Testosterone levels in IF were always considerably higher than those in SV blood, but this difference was not constant. Injection s.c. of rats with an LHRH agonist resulted in parallel increases in the serum levels of LH and in the IF and PV levels of testosterone. However, at 6 h there was an LH-independent secondary increase in testosterone levels which was associated with an increase in IF volume, reflecting an increase in capillary wall permeability and hence increased transport of LH into the testis.