Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing
Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing
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DOI：
10.1038/ng.259
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发表时间：
2008-12-01
期刊：
影响因子：
30.8
通讯作者：
Blencowe, Benjamin J.
中科院分区：
文献类型：
--
作者：
Pan, Qun;Shai, Ofer;Blencowe, Benjamin J.
我们首次使用mRNA-Seq数据分析了人体组织的选择性剪接复杂性。在大约20%的多外显子基因中检测到新的剪接连接，其中许多是组织特异性的。通过结合mRNA-Seq和EST-cDNA序列数据，我们估计大约95%的多外显子基因的转录本经历了选择性剪接，并且在人体主要组织中存在大约100,000个中高丰度的选择性剪接事件。通过与定量替代剪接微阵列分析数据的比较，我们还表明mRNA-Seq数据为外显子包含水平提供了可靠的测量。
We carried out the first analysis of alternative splicing complexity in human tissues using mRNA-Seq data. New splice junctions were detected in similar to 20% of multiexon genes, many of which are tissue specific. By combining mRNA-Seq and EST-cDNA sequence data, we estimate that transcripts from similar to 95% of multiexon genes undergo alternative splicing and that there are similar to 100,000 intermediate- to high-abundance alternative splicing events in major human tissues. From a comparison with quantitative alternative splicing microarray profiling data, we also show that mRNA-Seq data provide reliable measurements for exon inclusion levels.