Two migration inhibitory factors with different chromatographic behavior and isoelectric points.
Two migration inhibitory factors with different chromatographic behavior and isoelectric points.
复制标题
具有不同色谱行为和等电点的两种迁移抑制因子。
作者：
H. Remold;A. Mednis
通过Sephadex G-100凝胶过滤、蔗糖密度梯度电泳、等电聚焦等方法对豚鼠淋巴结细胞产生的迁移抑制因子（MIF）进行分离。鉴定并分离了两个不同的物种。一种是pH 3-MIF，等电点为3.0 ~ 4.5，从Sephadex G-75柱中洗脱，分子表观分子量为65,000 （Kd为0.05 ~ 0.12）。另一种是pH 5-MIF，其等电点为5.0至5.5，洗脱分子的表观分子量在25,000至43,000之间（Kd为0.15至0.23）。
Migration inhibitory factor (MIF), produced by stimulation of guinea pig lymph node cells with concanavalin A, was fractionated by Sephadex G-100 gel filtration, sucrose density gradient electrophoresis, and isoelectrofocussing. Two distinct species were identified and separated. One, pH 3-MIF, has an isoelectric point of 3.0 to 4.5 and elutes from Sephadex G-75 columns with molecules having an apparent m.w. of 65,000 (Kd of 0.05 to 0.12). The other, pH 5-MIF, has an isoelectric point of 5.0 to 5.5 and elutes with molecules having an apparent m.w. between 25,000 and 43,000 (Kd of 0.15 to 0.23).