SLIPPAGE SYNTHESIS OF SIMPLE SEQUENCE DNA
SLIPPAGE SYNTHESIS OF SIMPLE SEQUENCE DNA
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DOI：
10.1093/nar/20.2.211
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发表时间：
1992-01-25
影响因子：
14.9
通讯作者：
TAUTZ, D
中科院分区：
文献类型：
--
作者：
SCHLOTTERER, C;TAUTZ, D
简单序列DNA在体外的滑移合成的分析揭示了简单序列如何在体内产生的问题。我们表明，它是可能的，从短引物和聚合酶在体外合成所有类型的重复的二和三核苷酸基序。这种合成的速率取决于序列特异性滑动速率，但与合成片段的长度无关。这表明只有DNA片段的末端参与决定该速率，因此滑动是一种短程效应。滑动合成也发生在固定的模板上，其中只有一条链可以自由移动，这种情况类似于体内的染色体复制。因此，复制过程中的滑动很可能是观察到的种群个体之间简单序列延伸的长度多态性的原因。
The analysis of slippage synthesis of simple sequence DNA in vitro sheds some light on the question of how simple sequences arise in vivo. We show that it is possible to synthesize all types of repetitious di- and trinucleotide motifs starting from short primers and a polymerase in vitro. The rate of this synthesis depends on a sequence specific slippage rate, but is independent of the length of the fragments being synthesized. This indicates that only the ends of the DNA fragments are involved in determining this rate and that slippage is accordingly a short range effect. Slippage synthesis occurs also on a fixed template where only one strand is free to move, a situation which resembles chromosome replication in vivo. It seems therefore likely that slippage during replication is the cause of the observed length polymorphism of simple sequence stretches between individuals of a population.