水からの Vibrio anguillarum の検出
水からの Vibrio anguillarum の検出
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水中鳗弧菌的检测
DOI：
10.2331/suisan.52.641
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发表时间：
1986
影响因子：
0.4
通讯作者：
敏博 中井
中科院分区：
文献类型：
--
作者：
清邦 室賀;正彦 飯田;浩幸 松本;敏博 中井
采用碱性蛋白胨水和BTB teepole琼脂两步培养法，对香鱼Plecoglossus altivelis养殖池淡水和日本内海海水中的鳗弧菌进行了检测。结果，海水中尤其是水温低于20°C时经常检测到鳗弧菌，但尽管一些池塘出现了弧菌病，但淡水中却没有检测到鳗弧菌。在89株分离的鳗弧菌中，只有13株属于已知的A至I血清型，其中8株对香鱼或日本鳗鲡有致病性。体外实验表明，该生物体在海水中能持续存在，但在淡水中3至5小时内死亡。
Detections of Vibrio anguillarum from freshwater of ayu Plecoglossus altivelis culture ponds and sea water of the Inland Sea of Japan were made by a two-step culture method consisting of alkaline peptone water and BTB teepole agar. As the results, V. anguillarum was often detected from sea waters especially when water temperature was below 20°C, but not from freshwaters in spite of the occurrence of vibriosis in some ponds. Among 89 isolated strains of V. anguillarum, only 13 strains belong to the known serotypes form A to I, and 8 strains were pathogenic to ayu or Japanese eel Anguilla japonica. It was demonstrated by in vitro experiments that the organism persisted in sea water but perished within 3 to 5h in freshwater.