Gene rearrangements in gekkonid mitochondrial genomes with shuffling, loss, and reassignment of tRNA genes.
Gene rearrangements in gekkonid mitochondrial genomes with shuffling, loss, and reassignment of tRNA genes.
复制标题
DOI：
10.1186/1471-2164-15-930
复制
发表时间：
2014-10-24
期刊：
影响因子：
4.4
通讯作者：
Hashiguchi Y
中科院分区：
文献类型：
--
作者：
Kumazawa Y;Miura S;Yamada C;Hashiguchi Y
脊椎动物线粒体基因组是16-18 kbp的双链环状dna，编码一组37个基因。这些基因的排列和主要的非编码区在进化过程中相对保守，尽管在不同的谱系中已经描述了基因重排。串联重复-随机丢失模型已被用来解释大多数线粒体基因重排的机制。先前报道的壁虎有丝分裂基因组序列很少包括基因重排，我们在本研究中进行了探索。我们使用高通量测序方法从壁虎科中确定了7个新的有丝分裂基因组序列。tripolitanus Tropiocolotes有丝分裂基因组涉及基因块：tRNAArg， NADH脱氢酶亚基4L和NADH脱氢酶亚基4的串列复制。每个蛋白质编码基因的一个副本可能被假原化。tRNAArg基因的一个副本似乎在第二个反密码子位置通过C碱基到T碱基的替换转化为trnaln基因，尽管该基因在蛋白质合成中可能不完全起作用。窄指藻有丝分裂基因组包括tRNALeu基因的几个串联复制，tRNAAla基因的易位和tRNA基因簇内光链复制的假定起源。最后，尾盘鱼和乌氏乌氏有丝分裂基因组的特征是tRNAGlu基因从其原始位置明显丢失。棘尾虎似乎保留了一个易位的tRNAGlu基因，该基因位于主要非编码区5 '端附近。本研究描述了几个新的线粒体基因重排从壁虎科。tRNA基因的丢失和重新分配在脊椎动物有丝分裂基因组中并不常见，我们的发现提出了新的问题，即缺失的tRNA是如何被补充的，以及重新分配的tRNA基因是否具有完全的功能。这些壁虎线粒体基因重排的新例子应该拓宽我们对线粒体基因排列进化的理解。本文的在线版本（doi:10.1186/1471-2164-15-930）包含补充材料，可供授权用户使用。
Vertebrate mitochondrial genomes (mitogenomes) are 16–18 kbp double-stranded circular DNAs that encode a set of 37 genes. The arrangement of these genes and the major noncoding region is relatively conserved through evolution although gene rearrangements have been described for diverse lineages. The tandem duplication-random loss model has been invoked to explain the mechanisms of most mitochondrial gene rearrangements. Previously reported mitogenomic sequences for geckos rarely included gene rearrangements, which we explore in the present study. We determined seven new mitogenomic sequences from Gekkonidae using a high-throughput sequencing method. The Tropiocolotes tripolitanus mitogenome involves a tandem duplication of the gene block: tRNAArg, NADH dehydrogenase subunit 4L, and NADH dehydrogenase subunit 4. One of the duplicate copies for each protein-coding gene may be pseudogenized. A duplicate copy of the tRNAArg gene appears to have been converted to a tRNAGln gene by a C to T base substitution at the second anticodon position, although this gene may not be fully functional in protein synthesis. The Stenodactylus petrii mitogenome includes several tandem duplications of tRNALeu genes, as well as a translocation of the tRNAAla gene and a putative origin of light-strand replication within a tRNA gene cluster. Finally, the Uroplatus fimbriatus and U. ebenaui mitogenomes feature the apparent loss of the tRNAGlu gene from its original position. Uroplatus fimbriatus appears to retain a translocated tRNAGlu gene adjacent to the 5’ end of the major noncoding region. The present study describes several new mitochondrial gene rearrangements from Gekkonidae. The loss and reassignment of tRNA genes is not very common in vertebrate mitogenomes and our findings raise new questions as to how missing tRNAs are supplied and if the reassigned tRNA gene is fully functional. These new examples of mitochondrial gene rearrangements in geckos should broaden our understanding of the evolution of mitochondrial gene arrangements. The online version of this article (doi:10.1186/1471-2164-15-930) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
4.4
作者：
Kurabayashi A;Sumida M
通讯作者：
Sumida M
影响因子：
2.7
作者：
KUMAZAWA, Y;HIMENO, H;WATANABE, K
通讯作者：
WATANABE, K
影响因子：
3.5
作者：
Amer, SAM;Kumazawa, Y
通讯作者：
Kumazawa, Y
影响因子：
4.1
作者：
Bernt, Matthias;Bleidorn, Christoph;Struck, Torsten H.
通讯作者：
Struck, Torsten H.
影响因子：
64.8
作者：
Ito, Takuhiro;Yokoyama, Shigeyuki
通讯作者：
Yokoyama, Shigeyuki