Role of paraventricular nucleus in regulation of sympathetic nerve frequency components
Role of paraventricular nucleus in regulation of sympathetic nerve frequency components
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DOI：
10.1152/ajpheart.00673.2002
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发表时间：
2003-05-01
影响因子：
4.8
通讯作者：
Fels, RJ
中科院分区：
文献类型：
--
作者：
Kenney, MJ;Weiss, ML;Fels, RJ
用自谱分析和相干分析方法研究了室旁核（PVN）一氧化氮、γ-氨基丁酸（GABA）和N-甲基-D-天冬氨酸（NMDA）-谷氨酸受体在麻醉大鼠交感神经放电（SND）频率成分调节中的作用及其相互作用。提出了四点意见。首先，PVN微量注射荷包牡丹碱（BIC）（GABA（A）受体拮抗剂），而不是单一PVN注射NMDA（兴奋性氨基酸）或N-G-单甲基-L-精氨酸（L-NMMA;一氧化氮合酶抑制剂），改变SND频率成分。第二，联合PVN微量注射L-NMMA和NMDA改变了SND爆发模式，然而，观察到的模式变化是不同的PVN BIC产生的，没有观察到窦弓去神经后。第三，PVN微量注射犬尿烯酸预防和逆转BIC诱导的SND爆发模式的变化。最后，尽管肾脏和脾脏SND水平没有变化，但PVN BIC微量注射后血管阻力（肾脏和脾脏）显著增加。这些数据表明，PVN包含改变SND频率成分的神经基质，并表明特定PVN神经递质之间的复杂相互作用和PVN神经递质与SND调节中的动脉压力感受器反射之间的复杂相互作用。
Autospectral and coherence analyses were used to determine the role of and interactions between paraventricular nucleus (PVN) nitric oxide, gamma-aminobutyric acid ( GABA), and the N-methyl-D-aspartic acid (NMDA)-glutamate receptor in regulation of sympathetic nerve discharge (SND) frequency components in anesthetized rats. Four observations were made. First, PVN microinjection of bicuculline (BIC) (GABA(A) receptor antagonist), but not single PVN injections of NMDA ( excitatory amino acid) or N-G-monomethyl-L-arginine (L-NMMA; a nitric oxide synthase inhibitor), altered SND frequency components. Second, combined PVN microinjections of L-NMMA and NMDA changed the SND bursting pattern; however, the observed pattern change was different from that produced by PVN BIC and not observed after sinoaortic denervation. Third, PVN microinjection of kynurenic acid prevented and reversed BIC-induced changes in the SND bursting pattern. Finally, vascular resistance ( renal and splenic) was significantly increased after PVN BIC microinjection despite the lack of change in the level of renal and splenic SND. These data demonstrate that the PVN contains the neural substrate for altering SND frequency components and suggest complex interactions between specific PVN neurotransmitters and between PVN neurotransmitters and the arterial baroreceptor reflex in SND regulation.