Diversity of Anaplasma species in cattle in Mozambique
Diversity of Anaplasma species in cattle in Mozambique
复制标题
DOI：
10.1016/j.ttbdis.2019.02.012
复制
发表时间：
2019-04-01
影响因子：
3.2
通讯作者：
Andre, Marcos Rogerio
中科院分区：
文献类型：
--
作者：
Fernandes, Simone de Jesus;Matos, Carlos Antonio;Andre, Marcos Rogerio
尽管无形体是家养和野生反刍动物中高度流行的立克次氏体病原体，在世界范围内广泛分布，但迄今为止，很少有研究来检测和/或调查莫桑比克牛体内这些病原体的多样性。在本研究中，使用血清学和分子测定来研究无形体属的发生。在莫桑比克马普托的博阿内、马古德、马图图因、莫安巴和纳马查地区采样的 219 头牛中。在检测边缘曲霉IgG抗体的iELISA测试中，86.3%（189/219）的样本呈阳性。在对边缘曲霉的 msp1 beta 基因和嗜吞噬细胞曲霉的 msp2 基因进行 qPCR 检测时，分别有 97.3% (213/219) 和 2.7% (6/219) 的动物呈阳性。基于 16S rRNA 基因的两种不同的 cPCR 方案显示，100% 的样品对无形体属呈阳性。获得的DNA序列在系统发育上与A. platys、A. phagocytophilum、Candidatus Anaplasma boleense、A.centrale、A.marginale和A.ovis相关。基于 msp4 和 msp5 基因的系统发育推断将获得的序列定位在 A.marginale 的进化枝中，有证据表明每个基因分别出现 8 种和 5 种不同的单倍型。无形体基于 16S rRNA 和 groEL 基因的系统发育分析证明了与 A. platys 的系统发育相关性。结论是无形体物种具有高度多样性。发生在莫桑比克的牛身上。
Although species of Anaplasma are highly prevalent Rickettsiales agents in domestic and wild ruminants with a wide distribution worldwide, few studies have been conducted so far to detect and/or investigate the diversity of these agentsin cattle in Mozambique. In the present study, serological and molecular assays were used to investigate the occurrence of Anaplasma spp. in 219 bovines sampled in the districts of Boane, Magude, Matutuine, Moamba and Namaacha in Maputo, Mozambique. In the iELISA test for detection ofIgG antibodies to A. marginale, 86.3% (189/219) of the samples were positive. In qPCR assays for the gene msp1 beta for A. marginale and msp2 for A. phagocytophilum, 97.3% (213/219) and 2.7% (6/219) of the animals were positive, respectively. Two different cPCR protocols based on the 16S rRNA gene showed that 100% of the samples were positive for Anaplasma spp. The DNA sequences obtained were phylogenetically related to A. platys, A. phagocytophilum, Candidatus Anaplasma boleense, A. centrale, A. marginale and A. ovis. Phylogenetic inference based on the msp4 and msp5 genes positioned the obtained sequences in the clade of A. marginale, with evidence of occurrence of 8 and 5 different haplotypes for each gene, respectively. Anaplasma sp. phylogenetically associated with A. platys was evidenced in phylogenetic analyzes based on 16S rRNA and groEL genes. It is concluded that a high diversity of species of Anaplasma spp. occurs in cattle in Mozambique.