JAK/STAT signalling regulates antimicrobial activities in Eriocheir sinensis
JAK/STAT signalling regulates antimicrobial activities in Eriocheir sinensis
复制标题
JAK/STAT 信号调节中华绒螯蟹的抗菌活性
DOI：
10.1016/j.fsi.2018.09.059
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发表时间：
2019
影响因子：
4.7
通讯作者：
Wang Qun
中科院分区：
文献类型：
--
作者：
Ruan Ze-Chao;Wan Zhi-Cheng;Yang Lei;Li Wei-Wei;Wang Qun
The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signalling pathway plays a significant role in immune responses to pathogens. In invertebrates, three core components (Domeless, Hopscotch and STAT92E) of the JAK/STAT pathway were first identified inDrosophila melanogaster. In the present study, we report the cloning and characterisation of DOME, JAK and STAT fromEriocheir sinensis.EsDOME,EsJAK andEsSTAT are 1299, 1110 and 794 amino acid proteins encoded by 4200, 3333 and 2385 bp open reading frames, respectively. Bioinformatics analysis revealed that all three share specific domains with corresponding functions. Quantitative real-time PCR showed that all three components were highly expressed in various tissues including the gill, hepatopancreas and hemocytes. Moreover,EsDOME,EsJAK andEsSTAT were upregulated significantly in hemocytes after bacterial challenge. In addition, the JAK/STAT signalling pathway positively regulates antimicrobial peptide expression during the antibacterial immune response. Thus, the JAK/STAT pathway plays a critical role in bacterial immune responses inE. sinensis.
