Evaluation of the Cozart® RapiScan drug test system for opiates and cocaine in oral fluid
Evaluation of the Cozart® RapiScan drug test system for opiates and cocaine in oral fluid
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DOI：
10.1016/j.forsciint.2004.12.042
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发表时间：
2005-06-10
影响因子：
2.2
通讯作者：
Hand, C
中科院分区：
文献类型：
--
作者：
Cooper, G;Wilson, L;Hand, C
本研究的目的是评价Cozart（R）RapiScan（CRS）药物检测系统检测口腔液中阿片类药物和可卡因的效率。使用Cozart（R）RapiScan收集系统从358名正在接受成瘾治疗并监测药物滥用情况的捐赠者中收集了口腔液样本。另外103份口腔液样本是从非吸毒者的志愿捐献者中收集的。在实验室中使用用于阿片类药物和可卡因的双面板Cozart（R）RapiScan盒对样本进行分析，并使用气相色谱-质谱法（GC-MS）进行确认。样品冷冻保存在-20 ° C，直到通过GC-MS分析。阿片类药物和可卡因的CRS的总体准确度为100%。以高于和低于临界值50%加标的样品始终分别得到阴性和阳性结果。共有88个样本对各种阿片类药物呈阳性，111个样本对可卡因和/或其代谢物呈阳性。使用纯口腔液中30 ng/mL吗啡或苯甲酰芽子碱当量的截止值，口腔液中阿片类药物和可卡因的CRS与GC-MS相比，总体效率分别为98%和99%。对口腔液的一系列潜在掺杂物进行了评价，结果显示不会改变测试结果。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
The purpose of this study was to evaluate the efficiency of the Cozart (R) RapiScan (CRS) drug test system for detecting opiates and cocaine in oral fluid. Oral fluid samples were collected using the Cozart (R) RapiScan collection system from 358 donors who were receiving treatment for their addiction and were monitored for drug misuse. A further 103 oral fluid samples were collected from volunteer donors who were not drug users. The samples were analyzed in the laboratory using the two-panel Cozart (R) RapiScan cartridge for opiates and cocaine and confirmed using gas chromatography-mass spectrometry (GC-MS). The samples were stored frozen at -20 degrees C until analysis by GC-MS. The overall accuracy of the CRS for both opiates and cocaine was 100%. Samples spiked at 50% above and below the cut-off consistently gave negative and positive results respectively. A total of 88 samples were positive for various opiates and 111 samples were positive for cocaine and/or its metabolites. The CRS for opiates and cocaine in oral fluid, using a cut-off of 30 ng/mL morphine or benzoylecgonine equivalents in neat oral fluid, had overall efficiencies of 98% and 99%, respectively, versus GC-MS. A series of potential adulterants of oral fluid were evaluated and shown not to alter the outcome of the test result. (c) 2005 Elsevier Ireland Ltd. All rights reserved.