Caragana korshinskii phenylalanine ammonialyase is up-regulated in the phenylpropanoid biosynthesis pathway in response to drought stress
Caragana korshinskii phenylalanine ammonialyase is up-regulated in the phenylpropanoid biosynthesis pathway in response to drought stress
复制标题
柠条苯丙氨酸解氨酶在苯丙素生物合成途径中响应干旱胁迫而上调
DOI：
10.1080/13102818.2019.1623718
复制
发表时间：
2019-01-01
影响因子：
1.4
通讯作者：
Gong, Chunmei
中科院分区：
文献类型：
--
作者：
Liu, Furong;Xie, Lifang;Gong, Chunmei
摘要干旱是最严重的非生物胁迫之一，各种植物通过激活机制来减轻干旱造成的伤害，但目前还不清楚。柠条锦鸡儿（Caragana korshinskii）是我国西北半干旱地区的一种旱生灌木，具有较强的耐旱性。基于C.以黄土高原沿着降水梯度采集的柠条叶片为材料，采用趋势分析法对大部分差异表达基因进行了分析。基因本体论分析表明，“苯丙素生物合成过程→次级代谢物生物合成过程”术语是最重要的基因本体论，而基于京都基因百科全书和基因组的分析表明，次级代谢物的生物合成是一条重要的代谢途径。实时荧光定量聚合酶链反应和酶活性分析证实了苯丙氨酸解氨酶（PAL）基因在C.在田间和实验室条件下研究了干旱胁迫对柠条的影响。这些结果表明C.干旱胁迫下，柠条通过调节次生代谢来适应水分亏缺环境，激活PAL。因此，对这些数据的进一步研究，可以扩展目前对C. Korshinskii。
Abstract Drought is one of the most severe abiotic stresses, the damage due to which, various plant species mitigate by activating mechanisms that are not yet well understood. Caragana korshinskii is a xerophytic shrub found in the semi-arid regions of northwest China with high tolerance to several abiotic stresses, including drought. Based on the de novo transcriptome data from C. korshinskii leaflets collected along a precipitation gradient on the Loess Plateau (China), most of the differentially expressed genes were explored using trend analysis along the precipitation gradient. Gene ontological analysis showed that “phenylpropanoid biosynthesis process → secondary metabolite biosynthetic process” terms were the most significant gene ontologies, whereas Kyoto Encyclopedia of Genes and Genomes-based analysis indicated that the biosynthesis of secondary metabolites was a significant metabolic pathway. Real-time polymerase chain reaction and enzyme activity analyses confirmed the increased transcription of the phenylalanine ammonialyase (PAL) gene in C. korshinskii under drought stress in field and laboratory conditions. These results suggested that C. korshinskii adjusts its secondary metabolism to water-deficit environments and activates PAL by drought stress. Therefore, further studies on the obtained data can expand the current understanding of the molecular and genetic mechanisms responsible for the drought endurance in C. korshinskii.