Type II Hydride Transferases from Different Microorganisms Yield Nitrite and Diarylamines from Polynitroaromatic Compounds
Type II Hydride Transferases from Different Microorganisms Yield Nitrite and Diarylamines from Polynitroaromatic Compounds
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来自不同微生物的 II 型氢化物转移酶从多硝基芳香族化合物中产生亚硝酸盐和二芳基胺
DOI：
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发表时间：
2008
影响因子：
4.4
通讯作者：
J. Ramos
中科院分区：
文献类型：
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作者：
P. van Dillewijn;R. Wittich;Antonio Caballero;J. Ramos
摘要 恶臭假单胞菌的 XenB、阴沟杆菌的季戊四醇四硝酸酯还原酶和大肠杆菌的 N-乙基马来酰亚胺还原酶（黄素蛋白老黄酶家族的所有 II 型氢化物转移酶）的均质制剂均能还原多硝基芳香族化合物 2,4,6-三硝基甲苯 (TNT)。该化合物的还原产生羟氨基二硝基甲苯和迈森海默二氢络合物，它们在缩合时产生化学计量的亚硝酸盐和二芳基胺，这意味着 II 型氢化物转移酶负责 TNT 脱硝，这一过程对 TNT 修复具有重要的环境影响。
ABSTRACT Homogenous preparations of XenB of Pseudomonas putida, pentaerythritol tetranitrate reductase of Enterobacter cloacae, and N-ethylmaleimide reductase of Escherichia coli, all type II hydride transferases of the Old Yellow Enzyme family of flavoproteins, are shown to reduce the polynitroaromatic compound 2,4,6-trinitrotoluene (TNT). The reduction of this compound yields hydroxylaminodinitrotoluenes and Meisenheimer dihydride complexes, which, upon condensation, yield stoichiometric amounts of nitrite and diarylamines, implying that type II hydride transferases are responsible for TNT denitration, a process with important environmental implications for TNT remediation.