Analyses of phenotype and ARGOS and ASY1 expression in a ploidy Chinese cabbage series derived from one haploid.
Analyses of phenotype and ARGOS and ASY1 expression in a ploidy Chinese cabbage series derived from one haploid.
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DOI：
10.1270/jsbbs.66.161
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发表时间：
2016-03
期刊：
影响因子：
2.4
通讯作者：
Shen SX
中科院分区：
文献类型：
--
作者：
Gu AX;Zhao JJ;Li LM;Wang YH;Zhao YJ;Hua F;Xu YC;Shen SX
本研究的目的是提高我们对倍性水平如何影响大白菜（Brassica rapa L. ssp. pekinensis）表型和基因表达的理解。用0.2%秋水仙碱诱导单倍体植物(2n = 10)产生二倍体(2n = 20)和四倍体植物(2n = 40)。非整倍体（2n = 24）也是通过作为雌性的二倍体植物与四倍体植物之间的杂交获得的。通过染色体计数和流式细胞术鉴定所有植物的倍性水平。随着倍性水平从单倍体增加到二倍体，从非整倍体增加到四倍体，叶子和花瓣变得更大。类似地，ARGOS 的表达受到基因组大小的调节，与倍性水平平行增加。在四种倍性类型中，花蕾中的表达强于叶中。 ASY1 的表达也根据倍性水平而不同，在二倍体植物中最高，其次是四倍体。单倍体和非整倍体在减数分裂之前和之后两个阶段的表达相似，但在减数分裂期间单倍体的表达较高。当在不同时期的同一倍性类型的芽中进行比较时，减数分裂期间ASY1的表达明显高于减数分裂之前或之后。我们的研究证明了源自一个单倍体的倍性大白菜系列的产生和表型。该倍性系列中ARGOS和ASY1基因的表达受基因组大小的调节，且两个基因的调节模式不同。
The aim of this research was to improve our understanding of how ploidy level influences phenotype and gene expression in Chinese cabbage (Brassica rapa L. ssp. pekinensis). Haploid plants (2n = 10) was induced by 0.2% colchicine to produce diploid (2n = 20) and tetraploid plants (2n = 40). The aneuploid (2n = 24) was also obtained by hybridization between diploid plants as the female and tetraploid plants. The ploidy levels of all plants were identified through chromosome counts and flow cytometry. Leaves and petals became larger as the ploidy level increased from haploid to diploid, and from aneuploid to tetraploid. Similarly, expression of ARGOS was regulated by genome size, increasing in parallel with the level of ploidy. Among the four ploidy types, expression was stronger in the floral buds than in the leaves. Expression by ASY1 also differed according to ploidy level, being highest in diploid plants, followed in order by tetraploids. Expression was similar between haploids and aneuploids at two stages—prior to and after meiosis—but was higher in the haploids during meiosis. When buds were compared within the same ploidy type at different stages, ASY1 expression was obviously higher during meiosis than either before or after. Our study demonstrated the generation and phenotype of a ploidy Chinese cabbage series derived from one haploid. Expression of genes ARGOS and ASY1 were modulated by genome size in this ploidy series, and the regulated patterns of the two genes was different.