DETECTION OF HYDROXYL RADICALS IN RAT STRIATUM DURING TRANSIENT FOCAL CEREBRAL-ISCHEMIA - POSSIBLE IMPLICATION IN TISSUE-DAMAGE
DETECTION OF HYDROXYL RADICALS IN RAT STRIATUM DURING TRANSIENT FOCAL CEREBRAL-ISCHEMIA - POSSIBLE IMPLICATION IN TISSUE-DAMAGE
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DOI：
10.1016/0304-3940(95)11901-8
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发表时间：
1995-09-08
影响因子：
2.5
通讯作者：
PLOTKINE, M
中科院分区：
文献类型：
--
作者：
LANCELOT, E;CALLEBERT, J;PLOTKINE, M
由于越来越多的证据表明，缺血损伤后的再灌注阶段可能通过产生羟基自由基（OH - o -）而加重组织损伤，我们研究了这些氧自由基是否在短暂局灶性脑缺血期间在大鼠纹状体中产生。(哦)- o -。采用水杨酸羟基化技术结合腔内微透析对透析液样品进行检测。通过左大脑中动脉和颈总动脉串联闭塞（45分钟），再灌注实现缺血。(哦)- o -。在缺血和早期再灌注时均可形成。此外，缺血引起纹状体梗死的体积与(OH)- o -的量呈正相关。在缺血和再灌注时产生。综上所述，这些结果提供了细胞毒性(OH)- o -形成的证据。在大鼠纹状体中可能参与该结构的缺血性损伤。
As increasing arguments suggest that the reperfusion phase following an ischemic insult may aggravate tissue injury by yielding hydroxyl radicals ((OH)-O-.), we examined whether these oxyradicals are generated in rat striatum during transient focal cerebral ischemia. (OH)-O-. were detected in dialysate samples by intrastriatal microdialysis coupled with the technique of salicylate hydroxylation. Ischemia was achieved by tandem occlusion of the left middle cerebral artery and common carotid arteries (45 min) followed by reperfusion. An (OH)-O-. formation occurred both during ischemia and early reperfusion. Additionally, the volume of the striatal infarct induced by ischemia correlated positively with the amount of (OH)-O-. produced during ischemia and reperfusion. Taken together, these results provide evidence of the formation of cytotoxic (OH)-O-. in rat striatum which might participate in the ischemic injury of this structure.