Absorption of zinc ions dissolved from zinc oxide nanoparticles in the tobacco callus improves plant productivity
Absorption of zinc ions dissolved from zinc oxide nanoparticles in the tobacco callus improves plant productivity
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DOI：
10.1007/s11240-019-01636-0
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发表时间：
2019-06
期刊：
影响因子：
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通讯作者：
Shizue Yoshihara;Kasumi Yamamoto;Yoshino Nakajima;S. Takeda;Kensuke Kurahashi;H. Tokumoto
中科院分区：
文献类型：
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作者：
Shizue Yoshihara;Kasumi Yamamoto;Yoshino Nakajima;S. Takeda;Kensuke Kurahashi;H. Tokumoto
氧化锌纳米颗粒（ZnO）可溶于水，并能释放Zn2+，这是一种促进植物细胞生长的必需矿物质。将氧化锌NPs施用于烟草（Nicotiana tabacumcv）。在白光照射下，Samusun-NN愈伤组织的重量呈浓度依赖性增加。具体来说，叶绿素水平的增加是由蓝光触发并由ZnO NPs诱导的。mRNA-seq分析表明，在氧化锌NPs和白光照射下，烟草愈伤组织早期盐胁迫相关基因的表达出现了较大的波动。24 h后，细胞组分和生长相关基因的表达也出现波动。RT-qPCR分析显示，暴露氧化锌NPs 1天后，光合作用相关基因的表达水平增强。对照处理的愈伤组织锌含量为0.19 mg g−1干重，而粒径为2000 nm的ZnO散装颗粒（bp）培养的愈伤组织锌含量为2.59 mg g−1干重，粒径为34 nm的ZnO散装颗粒培养的愈伤组织锌含量为3.37 mg g−1干重。这些结果表明，ZnO颗粒向愈伤组织提供了大量的Zn，说明粒径越小，颗粒表面积越大，越能有效地溶解锌离子，向烟草愈伤组织细胞提供更多的锌离子，从而提高植株的生产力。
Zinc oxide (ZnO) nanoparticles (NPs) are soluble in water and can release Zn2+, an essential mineral that promotes the growth of plant cells. When ZnO NPs were administered to tobacco (Nicotiana tabacumcv. Samusun-NN) callus under white light irradiation, a concentration-dependent increase in weight was observed. Specifically, an increase in chlorophyll levels was triggered by blue light and induced by ZnO NPs. mRNA-seq analysis showed that during the early stages of tobacco callus exposure to ZnO NPs and white light irradiation, there was considerable fluctuation in the expression of genes related to salt stress. After 24 h, the expression of cellular component and growth-related genes also fluctuated. Analysis by RT-qPCR revealed that, after 1 day of ZnO NPs exposure, the expression levels of photosynthesis-related genes were enhanced. The Zn content of control-treated callus was 0.19 mg g−1dry weight, whereas that of callus cultured with the ZnO bulk particles (BPs), with a particle diameter of 2000 nm, was 2.59 mg g−1dry weight, and for callus cultured with ZnO NPs, with a particle diameter 34 nm, the Zn content was 3.37 mg g−1dry weight. These results indicate that ZnO particles supplied large amounts of Zn to the callus, suggesting that the smaller the particle size, the larger the surface area of particles dissolve zinc ions more efficiently and the more ions are supplied to tobacco callus cells, and resulting in an increase in plant productivity.