Molecular characterization of the intestinal microbiota in patients with and without abdominal bloating
Molecular characterization of the intestinal microbiota in patients with and without abdominal bloating
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DOI：
10.1152/ajpgi.00044.2015
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发表时间：
2016-03-15
影响因子：
4.5
通讯作者：
Ringel, Yehuda
中科院分区：
文献类型：
--
作者：
Ringel-Kulka, Tamar;Benson, Andrew K.;Ringel, Yehuda
最近的研究表明，肠易激综合征(IBS)患者和健康对照组(HC)的肠道微生物区系存在差异，提示肠道微生物区系在IBS的发病机制中起着一定的作用。微生物区系的改变也与腹胀的发病机制有关，腹胀是IBS的常见症状。我们调查了一组IBS和HC患者的肠道微生物区系、腹胀和肠道模式改变之间的关系。从新鲜粪便标本中扩增出16S rRNA基因，并用Roche-454钛化学试剂盒进行焦解测序。核心可测量微生物组(CMM)是在>；检测到的75%的样本中检测到的操作分类单元(OTU)，并通过线性判别分析(LDA)比较了组间CMM的组成特征。通过在种/OTUS或CMM属中的连续变化，通过LDA将IBS与HC区分开来。当根据腹胀症状和肠道特征进行细分时，相同的受试者彼此之间以及与HC之间也有很好的区分。方差分析显示，包括IBS伴和不伴腹胀的亚组以及基于肠道特征的亚型之间存在定量的物种/OTU差异。两组之间明显的LDA分化和显著的微生物分类群差异表明，微生物区系与IBS的腹胀症状和肠道特征显著相关。这些微生物区系的变化可能是IBS及其临床亚型的一个生物标志物，并提示肠道微生物区系在该病主要症状的发病机制中发挥了作用。
Recent studies have demonstrated differences in the intestinal microbiota between patients with irritable bowel syndrome (IBS) and healthy controls (HC), suggesting a role for the intestinal microbiota in the pathogenesis of IBS. Alterations in the microbiota have also been implicated in the pathogenesis of abdominal bloating, a commonly reported symptom in IBS. We investigated the relationship between the intestinal microbiota, abdominal bloating, and altered bowel patterns in a cohort of patients with IBS and HC. The 16S rRNA gene from fresh fecal samples was amplified and pyrosequenced by using Roche-454 Titanium chemistry. A Core Measurable Microbiome (CMM) was generated for Operational Taxonomic Unit (OTU) detected in > 75% of all samples and compositional features of CMM were compared between groups by Linear Discriminant Analysis (LDA). IBS differentiated from HC by LDA using continuous variation in the species/OTUs or the CMM genera. When subcategorized based on bloating symptoms and bowel characteristics, the same subjects were also well differentiated from one another and from HC. ANOVA analysis showed quantitative species/OTU differences between the subgroups including IBS with and without bloating, and subtypes based on bowel characteristics. The clear LDA differentiation and the significant microbial taxa differences between the groups imply a significant association of the microbiota with bloating symptoms and bowel characteristics in IBS. These changes in the microbiota may serve as a biomarker for IBS and its clinical subtypes and suggest a role for the intestinal microbiota in the pathogenesis of the main symptoms of the disorder.