Age-related increase in PKC gamma expression in the cochlear nucleus of hearing impaired C57BL/6J and BALB/c mice
Age-related increase in PKC gamma expression in the cochlear nucleus of hearing impaired C57BL/6J and BALB/c mice
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听力受损 C57BL/6J 和 BALB/c 小鼠耳蜗核中 PKC γ 表达与年龄相关的增加
DOI：
10.1016/j.jchemneu.2010.10.003
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发表时间：
2011-01-01
影响因子：
2.8
通讯作者：
Li, Yun-Qing
中科院分区：
文献类型：
--
作者：
Kou, Zhen-Zhen;Zhang, Yong;Li, Yun-Qing
细胞信号传导的依赖性改变与年龄相关性听力损失（老年性耳聋）的发病有关。蛋白激酶C γ亚型（PKC γ）是一种仅在中枢神经系统表达的PKC同工酶，但其在老年性耳聋中的作用尚不清楚。采用两种老年性聋动物模型（C57 BL/6 J株和BALB/c株），分别测定青年（2月龄）、成年（8月龄）和老年（24月龄）3组动物的听性脑干反应（ABR）听阈，并采用免疫组化、Western blotting和Real-Time PCR检测耳蜗核（CN）内PKC γ的定位和表达。结果表明，PKC γ免疫反应阳性神经元主要集中在背侧CN（DCN）的分子层和梭形层，其数量随增龄而显著增加（p < 0.05）。与2月龄小鼠相比，8月龄和24月龄小鼠CN中PKC γ的蛋白和mRNA表达均显著增加（p < 0.05）。因此，我们的研究结果表明，在这些小鼠中增加的PKC γ表达和年龄相关的听力损失之间的潜在联系，提示预防和治疗年龄相关的听觉障碍的新策略。(C)2010 Elsevier B. V.保留所有权利。
Age-dependent alteration in cellular signaling is implicated in the onset of age-related hearing loss (presbycusis). The gamma subtype of protein kinase C (PKC gamma) is a PKC isoenzyme exclusively expressed in central nervous system but its potential role in the presbycusis remains unclear. Using two presbycusis-like animal models (C57BL/6J strain and BALB/c strain), the auditory thresholds were assessed by auditory brainstem response (ABR) in young (2-month-old), adult (8-month-old) and old (24-month-old) groups, and the localization and expression of PKC gamma in the cochlear nucleus (CN) was examined by immunohistochemistry, Western blotting and Real-Time PCR. The results showed that PKC gamma immmunoreactive (-ir) neurons were mainly concentrated in the molecular layer and fusiform layer of the dorsal CN (DCN) and their number was increased significantly with aging (p < 0.05). Moreover, compared with 2-month-old mice, PKC gamma expression in the CN at both protein and mRNA levels was significantly increased in the 8-month-old mice and 24-month-old mice (p < 0.05). Thus our findings demonstrate a potential link between the increased PKC gamma expression and the age-related hearing loss in these mice, suggesting novel strategies for the prevention and therapy of age-associated auditory disorders. (C) 2010 Elsevier B.V. All rights reserved.