Influence of a polymerizable eugenol derivative on the antibacterial activity and wettability of a resin composite for intracanal post cementation and core build-up restoration
Influence of a polymerizable eugenol derivative on the antibacterial activity and wettability of a resin composite for intracanal post cementation and core build-up restoration
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DOI：
10.1016/j.dental.2016.04.001
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发表时间：
2016-07-01
期刊：
影响因子：
5
通讯作者：
Deb, S.
中科院分区：
文献类型：
--
作者：
Almaroof, A.;Niazi, S. A.;Deb, S.
目标。丁香酚被用于牙科，因为它能够抑制一系列微生物的生长，包括通常从感染的根管中分离出来的兼性厌氧菌。本研究的目的是评估含有丁香酚的聚合物衍生物丁香基甲基丙烯酸酯单体（EgMA）的实验复合材料对一系列口腔细菌的抗菌活性，这些细菌通常与冠状和牙髓修复失败有关。并对其体外复合性能、润湿性及抗菌活性进行了研究。以羟基磷灰石（HA）和氧化锆ZrO2为填料，将EgMA单体（5%和10%）分别加入到BisGMA/TEGDMA树脂基配方中。采用直接接触试验评价复合材料对粪肠球菌、变形链球菌和痤疮丙酸杆菌的抑菌活性，并与未添加EgMA的复合材料进行比较。为明确其抑菌方式，还进行了琼脂扩散试验（ADT）。吸水性、溶解度、扩散系数、接触角和表面自由能作为互补的临床相关性能进行了测定。吸附和润湿性研究表明，随着EgMA单体含量的增加，复合材料的吸水性和表面自由能值降低，导致复合材料的疏水性显著增加。由于没有任何可浸出的抗菌剂，在任何对三种细菌进行测试的复合材料中都没有检测到抑制区。具有复合表面的共价锚定EgMA单体通过减少三种细菌的cfu数量而显示出有效的抑菌活性，并且对EgMA浓度在5%和10%重量时没有显着依赖。实验复合材料对3种病原菌的表面抑菌活性R值不同，其值从大到小在2.7 ~ 6.1之间，依次为E. faecalis < S. mutans < P. acnees。EgMA单体在可聚合配方中的掺入提供了一种新的方法来产生本质抗菌树脂复合材料，用于不同的牙科应用。(C) 2016年牙科材料学会。Elsevier Ltd.出版。版权所有。
Objectives. Eugenol has been used in dentistry due to its ability to inhibit the growth of a range of microorganisms, including facultative anaerobes commonly isolated from infected root canals. The aim of this study was to evaluate the antibacterial activity of the experimental composites containing eugenyl methacrylate monomer (EgMA), a polymeric derivative of eugenol, against a range of oral bacteria, commonly associated with failure of coronal and endodontic restorations. In vitro composite behavior and wettability were also studied in conjunction with their antibacterial activity.Methods. EgMA monomer (5 and 10% by weight) was added into BisGMA/TEGDMA resin based formulations with filler mixtures of hydroxyapatite (HA) and zirconium oxide ZrO2. The antibacterial activity of the experimental composites against Enterococcus faecalis, Streptococcus mutans and Propionibacterium acnes were evaluated by direct contact test and compared with composite formulation without inclusion of EgMA. To clarify the antibacterial mode of action, agar diffusion test (ADT) was also performed. Water sorption, solubility, diffusion coefficient, contact angle and surface free energy as complementary clinically relevant properties were determined.Results. Water sorption and wettability studies showed reduction of water uptake and surface free energy values with increasing content of EgMA monomer, resulting in significant increase in the hydrophobicity of the composites. No inhibition zones were detected in any of the composites tested against the three bacteria employed as expected, due to the absence of any leachable antibacterial agent. The covalently anchored EgMA monomer with the composite surface exhibited an effective bacteriostatic activity by reducing the number of CFUs of the three species of bacteria tested with no significant dependence on the concentration of EgMA at 5 and 10% by weight. The surface antibacterial activity R of the experimental composites were different against the three tested species with values in the range 2.7-6.1 following the order E. faecalis < S. mutans < P. acnes.Significance. The incorporation of EgMA monomer within polymerizable formulations provides a novel approach to yield intrinsically antibacterial resin composites for different dental applications. (C) 2016 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.