Differential expression and sequence-specific interaction of karyopherin alpha with nuclear localization sequences
Differential expression and sequence-specific interaction of karyopherin alpha with nuclear localization sequences
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DOI：
10.1074/jbc.272.7.4310
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发表时间：
1997-02-14
影响因子：
4.8
通讯作者：
Cleaveland, JS
中科院分区：
文献类型：
--
作者：
Nadler, SG;Tritschler, D;Cleaveland, JS
核蛋白转运过程需要几种不同的蛋白质直接或间接地与核定位或靶向序列（NLS）相互作用。最近，已经鉴定了许多核细胞蛋白α或nls结合蛋白。我们发现核蛋白hSRP1和hSRP1 α在不同的白细胞细胞系中有差异表达，并可在正常的人外周血淋巴细胞中诱导表达。我们发现这两种核蛋白以不同的序列特异性方式结合不同的NLSs，并且序列特异性受其他细胞质蛋白的调节。不同NLSs与核蛋白cu的结合与它们进入细胞核的能力之间存在相关性。综上所述，这些数据为核进口过程的多级控制提供了证据。
The process of nuclear protein transport requires the interaction of several different proteins, either directly or indirectly with nuclear localization or targeting sequences (NLS). Recently, a number of karyopherins alpha, or NLS-binding proteins, have been identified. We have found that the karyopherins hSRP1 and hSRP1 alpha are differentially expressed in various leukocyte cell lines and could be induced in normal human peripheral blood lymphocytes, We show that the two karyopherins bind with varied specificities in a sequence specific manner to different NLSs and that the sequence specificity is modulated by other cytosolic proteins. There was a correlation between binding of karyopherins cu to different NLSs and their ability to be imported into the nucleus. Taken together, these data provide evidence for multiple levels of control of the nuclear import process.