Bioaugmentation with an acetate-oxidising consortium as a tool to tackle ammonia inhibition of anaerobic digestion
Bioaugmentation with an acetate-oxidising consortium as a tool to tackle ammonia inhibition of anaerobic digestion
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DOI：
10.1016/j.biortech.2013.07.041
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发表时间：
2013-10-01
影响因子：
11.4
通讯作者：
Angelidaki, Irini
中科院分区：
文献类型：
--
作者：
Fotidis, Ioannis A.;Karakashev, Dimitar;Angelidaki, Irini
氨是沼气厂厌氧消化过程的主要抑制剂。在本研究中，氨耐受性SAO共培养物（即，梭状芽孢杆菌属（Clostridium ultunense spp.）nov.与甲烷库菌属（Methanoculleus spp.）菌株MAB 1）在中温升流式厌氧污泥床（UASB）反应器中进行高氨负荷试验。同时，对一株产氢产甲烷菌（Methanoculleus bourgensis MS 2（T））与SAO共培养物在流加反应器中的共培养进行了研究。结果表明，在UASB反应器中SAO共培养物的生物强化是不可能的，最有可能是由于产甲烷伴侣引起的培养物的最大生长速率（mu（max）= 0.007 h（-1））缓慢。M.在补料分批反应器中，将Bourgensis转化为SAO使菌体生长速率提高了42%（mu（max）= 0.01 h（-1））。这表明产甲烷菌是SAO共培养物中最慢的伙伴，因此是UASB反应器中生物强化过程中的限制因素。(c)2013爱思唯尔有限公司保留所有权利。
Ammonia is the major inhibitor of anaerobic digestion (AD) process in biogas plants. In the current study, the bioaugmentation of the ammonia tolerant SAO co-culture (i.e. Clostridium ultunense spp. nov. in association with Methanoculleus spp. strain MAB1) in a mesophilic up-flow anaerobic sludge blanket (UASB) reactor subjected to high ammonia loads was tested. The co-cultivation in fed-batch reactors of a fast-growing hydrogenotrophic methanogen (i.e. Methanoculleus bourgensis MS2(T)) with the SAO co-culture was also investigated. Results demonstrated that bioaugmentation of SAO co-culture in a UASB reactor was not possible most likely due to the slow maximum growth rate (mu(max) = 0.007 h(-1)) of the culture caused by the methanogenic partner. The addition of M. bourgensis to SAO led to 42% higher growth rate (mu(max) = 0.01 h(-1)) in fed-batch reactors. This indicates that methanogens were the slowest partners of the SAO co-culture and therefore were the limiting factor during bioaugmentation in the UASB reactor. (c) 2013 Elsevier Ltd. All rights reserved.