Differential gene expression in ripening banana fruit
Differential gene expression in ripening banana fruit
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DOI：
10.1104/pp.115.2.463
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发表时间：
1997-10-01
期刊：
影响因子：
7.4
通讯作者：
May, GD
中科院分区：
文献类型：
--
作者：
Clendennen, SK;May, GD
香蕉（梅萨（Mesa acominata L.）果实成熟乙烯的产生触发了发育级联反应，伴随着淀粉向糖的大量转化、相关的呼吸活动的爆发以及蛋白质合成的增加。代表香蕉果肉成熟阶段1和3的cDNA文库的差异筛选导致了11个非冗余组的差异表达的mRNA的分离。通过部分序列分析鉴定这些转录本表明，其中两种mRNA编码参与碳水化合物代谢的蛋白质，而其他mRNA编码被认为与植物的发病机制、衰老或应激反应相关的蛋白质。它们在果肉中的相对丰度和温室生长的香蕉植物中的组织特异性分布通过Northern印迹分析来确定。淀粉合成酶，颗粒结合淀粉合成酶，几丁质酶，凝集素，和2型金属硫蛋白的相对丰度在果肉成熟过程中下降。转录编码内切几丁质酶，β-1，3-葡聚糖酶，奇异果甜蛋白样蛋白，抗坏血酸过氧化物酶，金属硫蛋白，和一个假定的衰老相关蛋白在成熟早期增加。阐明与香蕉成熟相关的分子事件将有助于更好地理解和控制这些过程，并将使我们能够实现在转基因香蕉植物中生产候选口服疫苗的长期目标。
During banana (Mesa acominata L.) fruit ripening ethylene production triggers a developmental cascade that is accompanied by a massive conversion of starch to sugars, an associated burst of respiratory activity, and an increase in protein synthesis. Differential screening of cDNA libraries representing banana pulp at ripening stages 1 and 3 has led to the isolation of 11 nonredundant groups of differentially expressed mRNAs. Identification of these transcripts by partial sequence analysis indicates that two of the mRNAs encode proteins involved in carbohydrate metabolism, whereas others encode proteins thought to be associated with pathogenesis, senescence, or stress responses in plants. Their relative abundance in the pulp and tissue-specific distribution in greenhouse-grown banana plants were determined by northern-blot analyses. The relative abundance of transcripts encoding starch synthase, granule-bound starch synthase, chitinase, lectin, and a type-2 metallothionein decreased in pulp during ripening. Transcripts encoding endochitinase, beta-1,3-glucanase, a thaumatin-like protein, ascorbate peroxidase, metallothionein, and a putative senescence-related protein increased early in ripening. The elucidation of the molecular events associated with banana ripening will facilitate a better understanding and control of these processes, and will allow us to attain our long-term goal of producing candidate oral vaccines in transgenic banana plants.