High-Fat Diet Induces Significant Metabolic Disorders in a Mouse Model of Polycystic Ovary Syndrome
High-Fat Diet Induces Significant Metabolic Disorders in a Mouse Model of Polycystic Ovary Syndrome
复制标题
高脂肪饮食会在多囊卵巢综合症小鼠模型中引起显着的代谢紊乱
DOI：
10.1095/biolreprod.114.120063
复制
发表时间：
2014-11-01
影响因子：
3.6
通讯作者：
Kang, Jihong
中科院分区：
文献类型：
--
作者：
Lai, Hao;Jia, Xiao;Kang, Jihong
多囊卵巢综合征（PCOS）是最常见的女性内分泌疾病，与生殖和代谢紊乱相关。脱氢表雄酮 (DHEA) 目前用于诱导 PCOS 小鼠模型。高脂肪饮食（HFD）已被证明会导致雌性小鼠肥胖和不孕。 HFD 对 DHEA 诱导的 PCOS 小鼠表型的可能影响尚不清楚。本研究的目的是研究以正常食物或 60% HFD 喂养的 DHEA 诱导的 PCOS 小鼠的生殖和代谢特征。正常饮食或 HFD 的青春期前 C57BL/6 小鼠（25 天）每天注射（皮下）载体芝麻油或 DHEA，连续 20 天。实验结束时，评估了生殖和代谢特征。我们的数据表明，HFD 不会影响 DHEA 治疗小鼠的生殖表型。然而，HFD 治疗导致 DHEA 治疗小鼠发生显着的代谢变化，包括肥胖、葡萄糖耐受不良、血脂异常和明显的肝脏脂肪变性。这些发现表明，HFD 在 DHEA 诱导的 PCOS 小鼠中诱导了独特的代谢特征。因此，DHEA 和 HFD 联合治疗可作为研究该综合征代谢紊乱机制的一种手段，特别是在患有 PCOS 的女性中肝脂肪变性的高患病率中。
Polycystic ovary syndrome (PCOS) is the most common female endocrinopathy associated with both reproductive and metabolic disorders. Dehydroepiandrosterone (DHEA) is currently used to induce a PCOS mouse model. High-fat diet (HFD) has been shown to cause obesity and infertility in female mice. The possible effect of an HFD on the phenotype of DHEA-induced PCOS mice is unknown. The aim of the present study was to investigate both reproductive and metabolic features of DHEA-induced PCOS mice fed a normal chow or a 60% HFD. Prepubertal C57BL/6 mice (age 25 days) on the normal chow or an HFD were injected (s.c.) daily with the vehicle sesame oil or DHEA for 20 consecutive days. At the end of the experiment, both reproductive and metabolic characteristics were assessed. Our data show that an HFD did not affect the reproductive phenotype of DHEA-treated mice. The treatment of HFD, however, caused significant metabolic alterations in DHEA-treated mice, including obesity, glucose intolerance, dyslipide-mia, and pronounced liver steatosis. These findings suggest that HFD induces distinct metabolic features in DHEA-induced PCOS mice. The combined DHEA and HFD treatment may thus serve as a means of studying the mechanisms involved in metabolic derangements of this syndrome, particularly in the high prevalence of hepatic steatosis in women with PCOS.