Regulation of vitamin D receptor and Genistein on bone metabolism in mouse osteoblasts and the molecular mechanism of osteoporosis.
Regulation of vitamin D receptor and Genistein on bone metabolism in mouse osteoblasts and the molecular mechanism of osteoporosis.
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发表时间：
2018-05
影响因子：
3.2
通讯作者：
C. Ye;Y. Pan;H. Zhou
中科院分区：
文献类型：
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作者：
C. Ye;Y. Pan;H. Zhou
本工作的目的是从细胞学和流行病学角度研究维生素D受体（VDR）和染料木黄酮（Gen）对植物雌激素骨代谢的调节机制。不同浓度Gen处理MC3T3-E1细胞，MTT比色法检测细胞增殖率。然后观察VDR受体阻断剂ZK159222对Gen效应的影响； MC3T3-E1细胞中加入Gen后，通过Western blotting检测VDR蛋白的表达。添加雌激素受体α阻滞剂MPP和雌激素受体β阻滞剂PHTPP后，观察Gen对VDR蛋白的调节作用。从早期流行病学调查的200名绝经后妇女的血液样本中提取DNA，观察每个样本中VDR基因Apa I和Bsm I的限制性片段长度多态性。使用饮食调查和骨矿物质密度检查对结果进行分析。结果表明，10-8mol/L Gen可以促进MC3T3-E1细胞的增殖（P小于0.05）。这种效果可以通过 VDR 阻断剂 ZK159222 来取消。通过分析VDR酶切位点的Apa I和Bsm I基因型，我们发现不同基因型之间的骨密度（BMD）没有显着差异（P>0.05）。此外，不同基因型膳食植物雌激素摄入量与BMD之间无显着相关性（P>0.05）。总之，VDR可以介导Gen对MC3T3-E1细胞增殖的影响。 Gen中VDR蛋白表达上调不是由雌激素受体介导的。而且VDR基因多态性与各部位BMD无关，与膳食植物雌激素摄入的骨代谢作用无关。
The aim of this work was to study the mechanisms of vitamin D receptor (VDR) and Genistein (Gen) on the regulation of bone metabolism of phytoestrogens from cellular and epidemiological perspectives. MC3T3-E1 cells were treated with different concentrations of Gen, and the cell-proliferation rate was detected by an MTT colorimetric assay. The effect of the VDR receptor blocker ZK159222 on the Gen effect was then observed; after adding Gen to MC3T3-E1 cells, we detected the expression of VDR protein via Western blotting. After adding estrogen receptor α-blocker MPP and estrogen receptor β-blocker PHTPP, we observed the effect of Gen on the regulation of the VDR protein. DNA was extracted from the blood samples of 200 postmenopausal women in the early epidemiological survey, and the restriction fragment length polymorphism of VDR gene Apa I and Bsm I in each sample was observed. The results were analyzed using dietary survey and bone mineral density examination. The results show that 10-8mol/L Gen can promote the proliferation of MC3T3-E1 cells (P less than 0.05). This effect can be canceled by the VDR blocker ZK159222. By analyzing the Apa I and Bsm I genotypes of VDR restriction sites, we discovered no significant difference in bone mineral density (BMD) between different genotypes (P>0.05). In addition, there was no significant correlation between dietary phytoestrogen intake and BMD in different genotypes (P>0.05). In conclusion, VDR can mediate the effect of Gen on the proliferation of MC3T3-E1 cells. The up-regulated expression of VDR protein in Gen is not mediated by the estrogen receptor. Moreover, the VDR gene polymorphism is not related to the BMD in various parts and is not related to the bone metabolism effect of the dietary plant estrogen intake.