gas7:: A gene expressed preferentially in growth-arrested fibroblasts and terminally differentiated Purkinje neurons affects neurite formation
gas7:: A gene expressed preferentially in growth-arrested fibroblasts and terminally differentiated Purkinje neurons affects neurite formation
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DOI：
10.1073/pnas.95.19.11423
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发表时间：
1998-09-15
影响因子：
11.1
通讯作者：
Lin-Chao, S
中科院分区：
文献类型：
--
作者：
Ju, YT;Chang, ACY;Lin-Chao, S
生长停滞特异性（气体）基因在进入静止状态的细胞中优先受到抑制。我们在血清饥饿的鼠成纤维细胞中鉴定的GAS 7在这里被报道在成熟的大脑皮层、海马和小脑的神经元细胞中选择性地体内表达。gas 7转录物编码一个48-kDa的蛋白质，该蛋白质含有一个类似于OCT 2序列的结构域，OCT 2是一种参与神经元发育的POU转录因子，突触蛋白在调节神经递质释放中起作用。使用原位杂交和免疫细胞化学分析，我们发现，GAS 7的表达发生显着的小脑浦肯野细胞和胚胎鼠小脑的终末分化培养的生产抑制阻碍成熟的浦肯野细胞的神经突生长，相反，GAS 7过度表达在未分化的神经母细胞瘤细胞培养显着促进神经突样生长。总的来说，我们的研究结果提供了这种气体基因的表达和神经元发育之间的关联的证据。
Growth arrest-specific (gas) genes are er,pressed preferentially in cells that enter a quiescent state. gas7, which we identified in serum-starved murine fibroblasts, is reported here to be expressed in vivo selectively in neuronal cells of the mature cerebral cortex, hippocampus, and cerebellum. gas7 transcripts encode a 48-kDa protein containing a structural domain that resembles sequences of OCT2, a POU transcription factor implicated in neuronal development, and synapsins, which have a role in modulating neurotransmitter release. Using iii situ hybridization and immunocytochemical analysis, we show that GAS7 expression occurs prominently in cerebellar Purkinje cells and that inhibition of production in terminally differentiating cultures of embryonic murine cerebellum impedes neurite outgrowth from maturing Purkinje cells, Conversely, GAS7 overexpression in undifferentiated neuroblastoma cell cultures dramatically promotes neurite-like outgrowth. Collectively, our results provide evidence for an association between expression of this gas gene and neuronal development.