INDEPENDENT SPATIAL WAVES OF BIOCHEMICAL DIFFERENTIATION ALONG THE SURFACE OF CHICKEN BRAIN AS REVEALED BY THE SEQUENTIAL EXPRESSION OF ACETYLCHOLINESTERASE
INDEPENDENT SPATIAL WAVES OF BIOCHEMICAL DIFFERENTIATION ALONG THE SURFACE OF CHICKEN BRAIN AS REVEALED BY THE SEQUENTIAL EXPRESSION OF ACETYLCHOLINESTERASE
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DOI：
10.1007/bf00214007
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发表时间：
1988-01-01
影响因子：
3.6
通讯作者：
HENGSTENBERG, R
中科院分区：
文献类型：
--
作者：
LAYER, PG;ROMMEL, S;HENGSTENBERG, R
乙酰胆碱酯酶阳性细胞突然聚集在神经上皮的浅层;这层最终覆盖，在一个鞘样的方式，整个表面的胚胎鸡脑。这一特征在功能上尚不清楚;然而，它出现在神经元有丝分裂后不久，并且这一层的侧向延伸可以很容易地在连续脑切片上使用组织化学追踪。因此，可以利用该层在空间上描绘生化组织分化的开始波。我们已经研究了整个大脑之间的阶段11和30，并提供了第一个完整的空间方案的大脑分化的基础上计算机重建，二维和三维地图。大脑并不区分为一个平滑的相干波，而是检测到五个独立的初级AChE激活区：第一个起源于第11阶段（“菱形脑波”），第二个同时起源（“中脑波”），第三个起源于第15阶段（“顶盖波”）。第四个区域在第18阶段从端脑的底部到顶部发育。视网膜发育也开始于第18阶段。在一个给定的区域，它似乎是乙酰胆碱酯酶的发展之前不久，形成的主要纤维束。因此，乙酰胆碱酯酶可能是纤维生长和寻路的先决条件。
AChE-positive cells suddenly amass in a superficial layer of the neuroepithelium; this layer finally covers, in a sheat-like manner, the entire surface of the embryonic chicken brain. This feature is functionally not understood; however, it appears shortly after the neurons become post-mitotic, and the lateral extensions of this layer can easily be traced using histochemistry on serial brain sections. The layer can therefore be exploited to delineate spatially the waves of onset of biochemical tissue differentiation. We have studied whole brains between stages 11 and 30 and provide the first complete spatial schemes of brain differentiation based on computer-reconstructed, two- and three-dimensional maps. The brain does not differentiate in one smooth coherent wave, but instead five separate primary AChE-activation zones are detected: the first originating at stage 11 (“rhombencephalic wave”), the second at the same time (“midbrain wave”), the third at stage 15 ("“tectal wave”). A fourth zone develops later, at stage 18, from the bottom part of the telencephalon to the top. Retinal development also starts at stage 18. In a given area, it appears that AChE-development shortly precedes that of the formation of major fiber tracts. AChE might therefore represent a prerequisite for fiber growth and pathfinding.